RNA splicing based on reporter genes system: Detection, imaging and applications

• Published in: Coordination chemistry reviews Vol. 477; p. 214929
• Main Authors: Shi, Xiaorui, Won, Miae, Tang, Chu, Ding, Qihang, Sharma, Amit, Wang, Fu, Kim, Jong Seung
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.02.2023
• Subjects: Biomedical application; Clinical translation; Fluorescence; Molecular imaging; Reporter genes; RNA splicing; Clk; HSV1-tk; TSTA; BFP; GLuc; MB; RT-PCR; b-gal; MDS; RFP; RNA splicing; AML; MBNL2; GUS; tRNA; CYBB; 5′SS; SDS-PAGE; Bla; MBNL; BTK; Reporter genes; hTERT; MAPT; MET; BLI; FDA; ORF; Ad; CELF; HTS; UAS; PTM; WAS; Fluorescence; Clinical translation; DM1; mRNA; CFP; TPI; snRNA; GFP; Cis-SAGe; RASONs; SMN2; snRNP; SSA; Molecular imaging; CGD; YFP; ISO; CCD; rRNA; VEGF; HPV-16; U2 AF; RLuc; 3′SS; 3D; pre-mRNA; PB; FLuc; Dlg4; IAS2; Biomedical application; FISH; cTNT; ISAR; mCKAP2; FGFR2; HF; PET; SMaRT

[Display omitted] •RNA splicing is an essential process that governs many aspects of gene expression.•The three main types of RNA splicing are pre-mRNA splicing, group Ⅰ and Ⅱ introns self-splicing.•Reporter system for visualization of RNA splicing mainly includes fluorescent and bioluminescent prot...