Biosynthesized Vitis vinifera seed gold nanoparticles induce apoptotic cell death in A431 skin cancer cells

Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and inert. In the present study, phytochemicals present in Vitis vinifera seeds were utilized as reducing agents for the synthesis of gold nanoparti...

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Published inRSC advances Vol. 6; no. 85; pp. 82205 - 82218
Main Authors Nirmala, J. Grace, Akila, S., Nadar, M. S. A. Muthukumar, Narendhirakannan, R. T., Chatterjee, Suvro
Format Journal Article
LanguageEnglish
Published 01.01.2016
Subjects
ambient temperature
antineoplastic agents
Apoptosis
Cancer
chemical constituents of plants
cytotoxicity
Fourier transform infrared spectroscopy
Gold
human cell lines
inhibitory concentration 50
Killing
light scattering
membrane potential
Membranes
mitochondrial membrane
nanogold
Nanoparticles
neoplasm cells
particle size
reducing agents
Seeds
skin neoplasms
Transmission electron microscopy
Vitis vinifera
X-ray diffraction
zeta potential
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Abstract Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and inert. In the present study, phytochemicals present in Vitis vinifera seeds were utilized as reducing agents for the synthesis of gold nanoparticles. The morphology, particle size and properties of V. vinifera gold nanoparticles (AuNPs) were characterized using transmission electron microscopy, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy and zeta potential. The nanoparticles were then incubated for 24 h with human epidermoid skin cancer cells (A431) and were evaluated for their antiproliferative activities and induction of apoptosis. Spherical gold nanoparticles of ∼50 ± 5 nm were synthesized at room temperature within ten min and were capped with phytochemicals from V. vinifera seeds. At the inhibitory concentration (IC 50 ) of V. vinifera seed AuNPs (24.2 μM), they imparted cytotoxic effects, increased the level of reactive oxygen species, and induced apoptosis and apoptotic morphological changes in A431 cells significantly ( p < 0.01); these effects are associated with interference in mitochondrial membrane potential. This reduction in mitochondrial membrane potential probably initiated the apoptotic cascade in the nanoparticle-treated cells. Thus, gold nanoparticles synthesized utilizing Vitis vinifera seed phytochemicals may selectively target cancer cells, and the phytochemicals that are occluded within the nanoparticles may serve as anticancer agents, providing greater efficacy in killing cancer cells.
AbstractList Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and inert. In the present study, phytochemicals present in Vitis vinifera seeds were utilized as reducing agents for the synthesis of gold nanoparticles. The morphology, particle size and properties of V. vinifera gold nanoparticles (AuNPs) were characterized using transmission electron microscopy, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy and zeta potential. The nanoparticles were then incubated for 24 h with human epidermoid skin cancer cells (A431) and were evaluated for their antiproliferative activities and induction of apoptosis. Spherical gold nanoparticles of similar to 50 plus or minus 5 nm were synthesized at room temperature within ten min and were capped with phytochemicals from V. vinifera seeds. At the inhibitory concentration (IC50) of V. vinifera seed AuNPs (24.2 mu M), they imparted cytotoxic effects, increased the level of reactive oxygen species, and induced apoptosis and apoptotic morphological changes in A431 cells significantly (p < 0.01); these effects are associated with interference in mitochondrial membrane potential. This reduction in mitochondrial membrane potential probably initiated the apoptotic cascade in the nanoparticle-treated cells. Thus, gold nanoparticles synthesized utilizing Vitis vinifera seed phytochemicals may selectively target cancer cells, and the phytochemicals that are occluded within the nanoparticles may serve as anticancer agents, providing greater efficacy in killing cancer cells.
Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and inert. In the present study, phytochemicals present in Vitis vinifera seeds were utilized as reducing agents for the synthesis of gold nanoparticles. The morphology, particle size and properties of V. vinifera gold nanoparticles (AuNPs) were characterized using transmission electron microscopy, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy and zeta potential. The nanoparticles were then incubated for 24 h with human epidermoid skin cancer cells (A431) and were evaluated for their antiproliferative activities and induction of apoptosis. Spherical gold nanoparticles of ∼50 ± 5 nm were synthesized at room temperature within ten min and were capped with phytochemicals from V. vinifera seeds. At the inhibitory concentration (IC₅₀) of V. vinifera seed AuNPs (24.2 μM), they imparted cytotoxic effects, increased the level of reactive oxygen species, and induced apoptosis and apoptotic morphological changes in A431 cells significantly (p < 0.01); these effects are associated with interference in mitochondrial membrane potential. This reduction in mitochondrial membrane potential probably initiated the apoptotic cascade in the nanoparticle-treated cells. Thus, gold nanoparticles synthesized utilizing Vitis vinifera seed phytochemicals may selectively target cancer cells, and the phytochemicals that are occluded within the nanoparticles may serve as anticancer agents, providing greater efficacy in killing cancer cells.
Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and inert. In the present study, phytochemicals present in Vitis vinifera seeds were utilized as reducing agents for the synthesis of gold nanoparticles. The morphology, particle size and properties of V. vinifera gold nanoparticles (AuNPs) were characterized using transmission electron microscopy, X-ray diffraction, dynamic light scattering, Fourier transform infrared spectroscopy and zeta potential. The nanoparticles were then incubated for 24 h with human epidermoid skin cancer cells (A431) and were evaluated for their antiproliferative activities and induction of apoptosis. Spherical gold nanoparticles of ∼50 ± 5 nm were synthesized at room temperature within ten min and were capped with phytochemicals from V. vinifera seeds. At the inhibitory concentration (IC 50 ) of V. vinifera seed AuNPs (24.2 μM), they imparted cytotoxic effects, increased the level of reactive oxygen species, and induced apoptosis and apoptotic morphological changes in A431 cells significantly ( p < 0.01); these effects are associated with interference in mitochondrial membrane potential. This reduction in mitochondrial membrane potential probably initiated the apoptotic cascade in the nanoparticle-treated cells. Thus, gold nanoparticles synthesized utilizing Vitis vinifera seed phytochemicals may selectively target cancer cells, and the phytochemicals that are occluded within the nanoparticles may serve as anticancer agents, providing greater efficacy in killing cancer cells.
Author Akila, S.
Narendhirakannan, R. T.
Nirmala, J. Grace
Nadar, M. S. A. Muthukumar
Chatterjee, Suvro
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Cites_doi 10.1021/la900680e
10.1016/j.jcis.2004.03.003
10.1038/nnano.2007.88
10.1016/j.aca.2008.10.002
10.2174/156652410792630634
10.1002/adfm.200500611
10.4103/0973-8398.100141
10.1007/s12645-011-0014-8
10.1016/j.lfs.2004.03.009
10.3390/ijms11020622
10.1093/carcin/23.11.1869
10.1016/j.matlet.2008.08.044
10.1038/cdd.2008.150
10.1016/S0300-483X(00)00210-9
10.1016/j.saa.2012.03.002
10.1186/1477-3155-10-17
10.1089/154065804322966315
10.1074/jbc.272.45.28191
10.1021/nl071008a
10.1016/j.etp.2008.09.001
10.1016/j.snb.2012.09.073
10.1080/00222930701314932
10.1007/s11051-008-9494-9
10.2217/17435889.2.1.113
10.1023/B:BREA.0000020991.55659.59
10.1111/j.1600-0625.2007.00542.x
10.1271/bbb.63.896
10.7314/APJCP.2014.15.10.4311
10.1016/j.nano.2007.03.005
10.1016/j.saa.2009.02.037
10.1016/j.cis.2010.02.001
10.1002/smll.200400093
10.1111/j.1349-7006.2010.01680.x
10.1186/1472-6750-5-12
10.1158/0008-5472.CAN-08-3978
10.1016/j.saa.2007.12.002
10.1039/c0nr00814a
10.1002/smll.200700378
10.1021/bm050902y
10.2174/157489209787002461
10.3109/17435390903471463
10.1023/A:1006926414683
10.1016/j.bbrc.2010.06.006
10.4143/crt.2009.41.1.1
10.1039/b9nr00112c
10.1016/0022-1759(83)90303-4
10.1182/blood-2002-08-2512
10.1016/j.drup.2009.02.002
10.1007/s00280-003-0726-5
10.3322/canjclin.51.1.15
10.1517/17425241003777010
10.1111/j.0022-202X.2004.22625.x
10.1111/j.1744-7348.2003.tb00278.x
10.1021/jp972807s
10.1016/j.freeradbiomed.2010.08.031
10.7314/APJCP.2013.14.4.2213
10.1007/978-0-387-76713-0_4
10.1088/0957-4484/23/45/455103
10.1016/j.canlet.2007.08.020
10.1021/nl052396o
10.1016/S0024-3205(03)00566-6
10.2147/NSA.S3707
10.1039/C4RA07285E
10.1002/biof.5520220109
10.1021/nl070363y
10.1038/nrc1736
10.1590/s1516-8913201500076
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References Greenlee (C6RA16310F-(cit1)/*[position()=1]) 2001; 51
Kim (C6RA16310F-(cit18)/*[position()=1]) 2009; 1
C6RA16310F-(cit54)/*[position()=1]
Qiao (C6RA16310F-(cit64)/*[position()=1]) 2009; 12
Wong (C6RA16310F-(cit6)/*[position()=1]) 2009; 4
Xu (C6RA16310F-(cit10)/*[position()=1]) 2006; 7
Chithrani (C6RA16310F-(cit56)/*[position()=1]) 2007; 7
Han (C6RA16310F-(cit17)/*[position()=1]) 2007; 2
Souquet (C6RA16310F-(cit23)/*[position()=1]) 2000; 73
Zhou (C6RA16310F-(cit59)/*[position()=1]) 2003; 101
Chithrani (C6RA16310F-(cit16)/*[position()=1]) 2006; 6
Narayanan (C6RA16310F-(cit53)/*[position()=1]) 2010; 156
Soppimath (C6RA16310F-(cit11)/*[position()=1]) 2007; 17
Jain (C6RA16310F-(cit14)/*[position()=1]) 2007; 7
Shankar (C6RA16310F-(cit52)/*[position()=1]) 2004; 275
Hug (C6RA16310F-(cit61)/*[position()=1]) 1997; 272
Sharma (C6RA16310F-(cit25)/*[position()=1]) 2004; 85
Ribble (C6RA16310F-(cit44)/*[position()=1]) 2005; 5
Han (C6RA16310F-(cit77)/*[position()=1]) 2007; 620
Mohansrinivasan (C6RA16310F-(cit31)/*[position()=1]) 2015; 58
Kroemer (C6RA16310F-(cit66)/*[position()=1]) 2009; 16
Arvizo (C6RA16310F-(cit4)/*[position()=1]) 2010; 7
Sastry (C6RA16310F-(cit51)/*[position()=1]) 2003; 85
Dash (C6RA16310F-(cit43)/*[position()=1]) 2013; 2013
Chen (C6RA16310F-(cit9)/*[position()=1]) 2008; 1
Engelbrecht (C6RA16310F-(cit28)/*[position()=1]) 2007; 258
Mironava (C6RA16310F-(cit75)/*[position()=1]) 2010; 4
Rahman (C6RA16310F-(cit76)/*[position()=1]) 2010; 23
Hileman (C6RA16310F-(cit57)/*[position()=1]) 2004; 53
El-Kassas (C6RA16310F-(cit78)/*[position()=1]) 2014; 15
Kabeer (C6RA16310F-(cit67)/*[position()=1]) 2012; 6
Mosmann (C6RA16310F-(cit39)/*[position()=1]) 1983; 65
Qiao (C6RA16310F-(cit7)/*[position()=1]) 2009; 12
Unfried (C6RA16310F-(cit63)/*[position()=1]) 2007; 1
Alias (C6RA16310F-(cit21)/*[position()=1]) 2009; 61
van Berlo (C6RA16310F-(cit3)/*[position()=1]) 2010; 49
Krishnaraj (C6RA16310F-(cit35)/*[position()=1]) 2012; 93
Seigneuric (C6RA16310F-(cit8)/*[position()=1]) 2010; 10
Newman (C6RA16310F-(cit33)/*[position()=1]) 2009; 25
Narayanan (C6RA16310F-(cit37)/*[position()=1]) 2008; 62
Nirmala (C6RA16310F-(cit45)/*[position()=1]) 2011; 3
Patra (C6RA16310F-(cit40)/*[position()=1]) 2007; 3
Xiao (C6RA16310F-(cit15)/*[position()=1]) 2011; 3
Nishiyama (C6RA16310F-(cit71)/*[position()=1]) 2007; 2
Taleb (C6RA16310F-(cit34)/*[position()=1]) 1998; 102
Mukherjee (C6RA16310F-(cit46)/*[position()=1]) 2012; 23
Meeran (C6RA16310F-(cit27)/*[position()=1]) 2007; 16
Philip (C6RA16310F-(cit49)/*[position()=1]) 2009; 73
Novak (C6RA16310F-(cit24)/*[position()=1]) 2008; 630
Rastogi (C6RA16310F-(cit41)/*[position()=1]) 2010; 397
Kasthuri (C6RA16310F-(cit36)/*[position()=1]) 2009; 11
Rahaman Mollick (C6RA16310F-(cit60)/*[position()=1]) 2014; 4
Singleton (C6RA16310F-(cit22)/*[position()=1]) 1992
Kawaii (C6RA16310F-(cit55)/*[position()=1]) 1999; 63
Tsang (C6RA16310F-(cit62)/*[position()=1]) 2003; 73
Riss (C6RA16310F-(cit58)/*[position()=1]) 2004; 2
Xia (C6RA16310F-(cit32)/*[position()=1]) 2010; 11
Zeng (C6RA16310F-(cit47)/*[position()=1]) 2013; 176
Bagchi (C6RA16310F-(cit30)/*[position()=1]) 2000; 148
Wang (C6RA16310F-(cit12)/*[position()=1]) 2009; 41
Muthuirulappan (C6RA16310F-(cit19)/*[position()=1]) 2013; 14
Siddiqui (C6RA16310F-(cit72)/*[position()=1]) 2009; 69
Raghunandan (C6RA16310F-(cit73)/*[position()=1]) 2011; 2
Ohigashi (C6RA16310F-(cit5)/*[position()=1]) 2004; 22
Zhang (C6RA16310F-(cit69)/*[position()=1]) 2010; 101
Agarwal (C6RA16310F-(cit29)/*[position()=1]) 2002; 23
Smitha (C6RA16310F-(cit48)/*[position()=1]) 2008; 71
Elia (C6RA16310F-(cit50)/*[position()=1]) 2014; 9
Pan (C6RA16310F-(cit74)/*[position()=1]) 2007; 3
Black (C6RA16310F-(cit2)/*[position()=1]) 2004; 122
Fesik (C6RA16310F-(cit65)/*[position()=1]) 2005; 5
Ghosh (C6RA16310F-(cit38)/*[position()=1]) 2012; 10
Cheng (C6RA16310F-(cit42)/*[position()=1]) 2004; 75
Ismail (C6RA16310F-(cit70)/*[position()=1]) 2014; 3
Connor (C6RA16310F-(cit13)/*[position()=1]) 2005; 1
Cragg (C6RA16310F-(cit20)/*[position()=1]) 2003; 143
Ye (C6RA16310F-(cit26)/*[position()=1]) 1999; 196
Singh (C6RA16310F-(cit68)/*[position()=1]) 2014; S5
References_xml – volume: 25
  start-page: 8993
  year: 2009
  ident: C6RA16310F-(cit33)/*[position()=1]
  publication-title: Langmuir
  doi: 10.1021/la900680e
– ident: C6RA16310F-(cit54)/*[position()=1]
– volume: 275
  start-page: 496
  year: 2004
  ident: C6RA16310F-(cit52)/*[position()=1]
  publication-title: J. Colloid Interface Sci.
  doi: 10.1016/j.jcis.2004.03.003
– volume: 2
  start-page: 203
  year: 2007
  ident: C6RA16310F-(cit71)/*[position()=1]
  publication-title: Nat. Nanotechnol.
  doi: 10.1038/nnano.2007.88
– volume: 630
  start-page: 107
  year: 2008
  ident: C6RA16310F-(cit24)/*[position()=1]
  publication-title: Anal. Chim. Acta
  doi: 10.1016/j.aca.2008.10.002
– volume: 10
  start-page: 640
  year: 2010
  ident: C6RA16310F-(cit8)/*[position()=1]
  publication-title: Curr. Mol. Med.
  doi: 10.2174/156652410792630634
– volume: 17
  start-page: 355
  year: 2007
  ident: C6RA16310F-(cit11)/*[position()=1]
  publication-title: Adv. Funct. Mater.
  doi: 10.1002/adfm.200500611
– volume: 6
  start-page: 51
  year: 2012
  ident: C6RA16310F-(cit67)/*[position()=1]
  publication-title: Asian J. Pharm. Clin. Res.
  doi: 10.4103/0973-8398.100141
– volume: 2
  start-page: 57
  year: 2011
  ident: C6RA16310F-(cit73)/*[position()=1]
  publication-title: Cancer Nanotechnol.
  doi: 10.1007/s12645-011-0014-8
– volume: 75
  start-page: 1579
  year: 2004
  ident: C6RA16310F-(cit42)/*[position()=1]
  publication-title: Life Sci.
  doi: 10.1016/j.lfs.2004.03.009
– volume: 11
  start-page: 622
  year: 2010
  ident: C6RA16310F-(cit32)/*[position()=1]
  publication-title: Int. J. Mol. Sci.
  doi: 10.3390/ijms11020622
– volume: 23
  start-page: 1869
  year: 2002
  ident: C6RA16310F-(cit29)/*[position()=1]
  publication-title: Carcinogenesis
  doi: 10.1093/carcin/23.11.1869
– volume: 62
  start-page: 4588
  year: 2008
  ident: C6RA16310F-(cit37)/*[position()=1]
  publication-title: Mater. Lett.
  doi: 10.1016/j.matlet.2008.08.044
– volume: 16
  start-page: 3
  year: 2009
  ident: C6RA16310F-(cit66)/*[position()=1]
  publication-title: Cell Death Differ.
  doi: 10.1038/cdd.2008.150
– volume: 148
  start-page: 187
  year: 2000
  ident: C6RA16310F-(cit30)/*[position()=1]
  publication-title: Toxicology
  doi: 10.1016/S0300-483X(00)00210-9
– volume: 93
  start-page: 95
  year: 2012
  ident: C6RA16310F-(cit35)/*[position()=1]
  publication-title: Spectrochim. Acta, Part A
  doi: 10.1016/j.saa.2012.03.002
– volume: 10
  start-page: 17
  year: 2012
  ident: C6RA16310F-(cit38)/*[position()=1]
  publication-title: J. Nanobiotechnol.
  doi: 10.1186/1477-3155-10-17
– volume: 2
  start-page: 51
  year: 2004
  ident: C6RA16310F-(cit58)/*[position()=1]
  publication-title: Assay Drug Dev. Technol.
  doi: 10.1089/154065804322966315
– volume: 272
  start-page: 28191
  year: 1997
  ident: C6RA16310F-(cit61)/*[position()=1]
  publication-title: J. Biol. Chem.
  doi: 10.1074/jbc.272.45.28191
– volume: 7
  start-page: 2080
  year: 2007
  ident: C6RA16310F-(cit14)/*[position()=1]
  publication-title: Nano Lett.
  doi: 10.1021/nl071008a
– volume: 2013
  start-page: 709269
  year: 2013
  ident: C6RA16310F-(cit43)/*[position()=1]
  publication-title: ISRN Oncol.
– volume: 61
  start-page: 205
  year: 2009
  ident: C6RA16310F-(cit21)/*[position()=1]
  publication-title: Exp. Toxicol. Pathol.
  doi: 10.1016/j.etp.2008.09.001
– volume: 176
  start-page: 1128
  year: 2013
  ident: C6RA16310F-(cit47)/*[position()=1]
  publication-title: Sens. Actuators, B
  doi: 10.1016/j.snb.2012.09.073
– volume: 1
  start-page: 52
  year: 2007
  ident: C6RA16310F-(cit63)/*[position()=1]
  publication-title: Nanotoxicology
  doi: 10.1080/00222930701314932
– volume: 11
  start-page: 1075
  year: 2009
  ident: C6RA16310F-(cit36)/*[position()=1]
  publication-title: J. Nanopart. Res.
  doi: 10.1007/s11051-008-9494-9
– volume: 2
  start-page: 113
  year: 2007
  ident: C6RA16310F-(cit17)/*[position()=1]
  publication-title: Nanomedicine
  doi: 10.2217/17435889.2.1.113
– volume: 85
  start-page: 1
  year: 2004
  ident: C6RA16310F-(cit25)/*[position()=1]
  publication-title: Breast Cancer Res. Treat.
  doi: 10.1023/B:BREA.0000020991.55659.59
– volume: 16
  start-page: 405
  year: 2007
  ident: C6RA16310F-(cit27)/*[position()=1]
  publication-title: Exp. Dermatol.
  doi: 10.1111/j.1600-0625.2007.00542.x
– volume: 63
  start-page: 896
  year: 1999
  ident: C6RA16310F-(cit55)/*[position()=1]
  publication-title: Biosci., Biotechnol., Biochem.
  doi: 10.1271/bbb.63.896
– volume: 15
  start-page: 4311
  year: 2014
  ident: C6RA16310F-(cit78)/*[position()=1]
  publication-title: Asian Pac. J. Cancer Prev.
  doi: 10.7314/APJCP.2014.15.10.4311
– volume: 3
  start-page: 111
  year: 2007
  ident: C6RA16310F-(cit40)/*[position()=1]
  publication-title: Nanomedicine
  doi: 10.1016/j.nano.2007.03.005
– volume: 73
  start-page: 374
  year: 2009
  ident: C6RA16310F-(cit49)/*[position()=1]
  publication-title: Spectrochim. Acta, Part A
  doi: 10.1016/j.saa.2009.02.037
– volume: 156
  start-page: 1
  year: 2010
  ident: C6RA16310F-(cit53)/*[position()=1]
  publication-title: Adv. Colloid Interface Sci.
  doi: 10.1016/j.cis.2010.02.001
– volume: 1
  start-page: 325
  year: 2005
  ident: C6RA16310F-(cit13)/*[position()=1]
  publication-title: Small
  doi: 10.1002/smll.200400093
– volume: 101
  start-page: 2375
  year: 2010
  ident: C6RA16310F-(cit69)/*[position()=1]
  publication-title: Cancer Sci.
  doi: 10.1111/j.1349-7006.2010.01680.x
– volume: 5
  start-page: 12
  year: 2005
  ident: C6RA16310F-(cit44)/*[position()=1]
  publication-title: BMC Biotechnol.
  doi: 10.1186/1472-6750-5-12
– volume: 69
  start-page: 1712
  year: 2009
  ident: C6RA16310F-(cit72)/*[position()=1]
  publication-title: Cancer Res.
  doi: 10.1158/0008-5472.CAN-08-3978
– volume: 71
  start-page: 186
  year: 2008
  ident: C6RA16310F-(cit48)/*[position()=1]
  publication-title: Spectrochim. Acta, Part A
  doi: 10.1016/j.saa.2007.12.002
– volume: 3
  start-page: 1383
  year: 2011
  ident: C6RA16310F-(cit15)/*[position()=1]
  publication-title: Nanoscale
  doi: 10.1039/c0nr00814a
– volume: 3
  start-page: 1941
  year: 2007
  ident: C6RA16310F-(cit74)/*[position()=1]
  publication-title: Small
  doi: 10.1002/smll.200700378
– volume: 7
  start-page: 829
  year: 2006
  ident: C6RA16310F-(cit10)/*[position()=1]
  publication-title: Biomacromolecules
  doi: 10.1021/bm050902y
– volume: 4
  start-page: 28
  year: 2009
  ident: C6RA16310F-(cit6)/*[position()=1]
  publication-title: Recent Pat. Anti-Cancer Drug Discovery
  doi: 10.2174/157489209787002461
– volume: 4
  start-page: 120
  year: 2010
  ident: C6RA16310F-(cit75)/*[position()=1]
  publication-title: Nanotoxicology
  doi: 10.3109/17435390903471463
– volume: 196
  start-page: 99
  year: 1999
  ident: C6RA16310F-(cit26)/*[position()=1]
  publication-title: Mol. Cell. Biochem.
  doi: 10.1023/A:1006926414683
– volume: 397
  start-page: 603
  year: 2010
  ident: C6RA16310F-(cit41)/*[position()=1]
  publication-title: Biochem. Biophys. Res. Commun.
  doi: 10.1016/j.bbrc.2010.06.006
– volume: 41
  start-page: 1
  year: 2009
  ident: C6RA16310F-(cit12)/*[position()=1]
  publication-title: Cancer Res. Treat.
  doi: 10.4143/crt.2009.41.1.1
– volume: 1
  start-page: 61
  year: 2009
  ident: C6RA16310F-(cit18)/*[position()=1]
  publication-title: Nanoscale
  doi: 10.1039/b9nr00112c
– volume: 65
  start-page: 55
  year: 1983
  ident: C6RA16310F-(cit39)/*[position()=1]
  publication-title: J. Immunol. Methods
  doi: 10.1016/0022-1759(83)90303-4
– volume: 85
  start-page: 162
  year: 2003
  ident: C6RA16310F-(cit51)/*[position()=1]
  publication-title: Curr. Sci.
– volume: 101
  start-page: 4098
  year: 2003
  ident: C6RA16310F-(cit59)/*[position()=1]
  publication-title: Blood
  doi: 10.1182/blood-2002-08-2512
– volume: 12
  start-page: 55
  year: 2009
  ident: C6RA16310F-(cit64)/*[position()=1]
  publication-title: Drug Resist. Updates
  doi: 10.1016/j.drup.2009.02.002
– volume: 53
  start-page: 209
  year: 2004
  ident: C6RA16310F-(cit57)/*[position()=1]
  publication-title: Cancer Chemother. Pharmacol.
  doi: 10.1007/s00280-003-0726-5
– volume: S5
  start-page: 009
  year: 2014
  ident: C6RA16310F-(cit68)/*[position()=1]
  publication-title: J. Nanomed. Nanotechnol.
– volume: 51
  start-page: 15
  year: 2001
  ident: C6RA16310F-(cit1)/*[position()=1]
  publication-title: Ca-Cancer J. Clin.
  doi: 10.3322/canjclin.51.1.15
– volume: 7
  start-page: 753
  year: 2010
  ident: C6RA16310F-(cit4)/*[position()=1]
  publication-title: Expert Opin. Drug Delivery
  doi: 10.1517/17425241003777010
– volume: 122
  start-page: xiii
  year: 2004
  ident: C6RA16310F-(cit2)/*[position()=1]
  publication-title: J. Invest. Dermatol.
  doi: 10.1111/j.0022-202X.2004.22625.x
– volume-title: Plant Polyphenols
  year: 1992
  ident: C6RA16310F-(cit22)/*[position()=1]
– volume: 143
  start-page: 127
  year: 2003
  ident: C6RA16310F-(cit20)/*[position()=1]
  publication-title: Ann. Appl. Biol.
  doi: 10.1111/j.1744-7348.2003.tb00278.x
– volume: 9
  start-page: 4007
  year: 2014
  ident: C6RA16310F-(cit50)/*[position()=1]
  publication-title: Int. J. Nanomed.
– volume: 102
  start-page: 2214
  year: 1998
  ident: C6RA16310F-(cit34)/*[position()=1]
  publication-title: J. Phys. Chem. B
  doi: 10.1021/jp972807s
– volume: 49
  start-page: 1685
  year: 2010
  ident: C6RA16310F-(cit3)/*[position()=1]
  publication-title: Free Radical Biol. Med.
  doi: 10.1016/j.freeradbiomed.2010.08.031
– volume: 14
  start-page: 2213
  year: 2013
  ident: C6RA16310F-(cit19)/*[position()=1]
  publication-title: Asian Pac. J. Cancer Prev.
  doi: 10.7314/APJCP.2013.14.4.2213
– volume: 620
  start-page: 48
  year: 2007
  ident: C6RA16310F-(cit77)/*[position()=1]
  publication-title: Adv. Exp. Med. Biol.
  doi: 10.1007/978-0-387-76713-0_4
– volume: 3
  start-page: 242
  year: 2011
  ident: C6RA16310F-(cit45)/*[position()=1]
  publication-title: Int. J. Pharm. Pharm. Sci.
– volume: 23
  start-page: 455103
  year: 2012
  ident: C6RA16310F-(cit46)/*[position()=1]
  publication-title: Nanotechnology
  doi: 10.1088/0957-4484/23/45/455103
– volume: 23
  start-page: 256
  year: 2010
  ident: C6RA16310F-(cit76)/*[position()=1]
  publication-title: Pak. J. Pharm. Sci.
– volume: 258
  start-page: 144
  year: 2007
  ident: C6RA16310F-(cit28)/*[position()=1]
  publication-title: Cancer Lett.
  doi: 10.1016/j.canlet.2007.08.020
– volume: 12
  start-page: 55
  year: 2009
  ident: C6RA16310F-(cit7)/*[position()=1]
  publication-title: Drug Resist. Updates
  doi: 10.1016/j.drup.2009.02.002
– volume: 73
  start-page: 835
  year: 2000
  ident: C6RA16310F-(cit23)/*[position()=1]
  publication-title: Bulletin of the International Organisation of Vine and Wine
– volume: 6
  start-page: 662
  year: 2006
  ident: C6RA16310F-(cit16)/*[position()=1]
  publication-title: Nano Lett.
  doi: 10.1021/nl052396o
– volume: 73
  start-page: 2047
  year: 2003
  ident: C6RA16310F-(cit62)/*[position()=1]
  publication-title: Life Sci.
  doi: 10.1016/S0024-3205(03)00566-6
– volume: 1
  start-page: 45
  year: 2008
  ident: C6RA16310F-(cit9)/*[position()=1]
  publication-title: Nanotechnol. Sci. Appl.
  doi: 10.2147/NSA.S3707
– volume: 4
  start-page: 37838
  year: 2014
  ident: C6RA16310F-(cit60)/*[position()=1]
  publication-title: RSC Adv.
  doi: 10.1039/C4RA07285E
– volume: 3
  start-page: 1
  year: 2014
  ident: C6RA16310F-(cit70)/*[position()=1]
  publication-title: Progress in Nanotechnology and Nanomaterials
– volume: 22
  start-page: 49
  year: 2004
  ident: C6RA16310F-(cit5)/*[position()=1]
  publication-title: BioFactors
  doi: 10.1002/biof.5520220109
– volume: 7
  start-page: 1542
  year: 2007
  ident: C6RA16310F-(cit56)/*[position()=1]
  publication-title: Nano Lett.
  doi: 10.1021/nl070363y
– volume: 5
  start-page: 876
  year: 2005
  ident: C6RA16310F-(cit65)/*[position()=1]
  publication-title: Nat. Rev. Cancer
  doi: 10.1038/nrc1736
– volume: 58
  start-page: 540
  year: 2015
  ident: C6RA16310F-(cit31)/*[position()=1]
  publication-title: Braz. Arch. Biol. Technol.
  doi: 10.1590/s1516-8913201500076
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Snippet Biologically synthesized gold nanoparticles provide many useful characteristics and can be used as carriers to deliver drugs because they are non-toxic and...
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SubjectTerms ambient temperature
antineoplastic agents
Apoptosis
Cancer
chemical constituents of plants
cytotoxicity
Fourier transform infrared spectroscopy
Gold
human cell lines
inhibitory concentration 50
Killing
light scattering
membrane potential
Membranes
mitochondrial membrane
nanogold
Nanoparticles
neoplasm cells
particle size
reducing agents
Seeds
skin neoplasms
Transmission electron microscopy
Vitis vinifera
X-ray diffraction
zeta potential
Title Biosynthesized Vitis vinifera seed gold nanoparticles induce apoptotic cell death in A431 skin cancer cells
URI https://www.proquest.com/docview/1845795703
https://www.proquest.com/docview/2253205282
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