A new dominant peroxiredoxin allele identified by whole-genome re-sequencing of random mutagenized yeast causes oxidant-resistance and premature aging

• Published in: Aging (Albany, NY.) Vol. 2; no. 8; pp. 475 - 486
• Main Authors: Timmermann, Bernd, Jarolim, Stefanie, Russmayer, Hannes, Kerick, Martin, Michel, Steve, Krüger, Antje, Bluemlein, Katharina, Laun, Peter, Grillari, Johannes, Lehrach, Hans, Breitenbach, Michael, Ralser, Markus
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 13.08.2010
• Subjects: Alleles; Animals; Base Sequence; Benzene Derivatives - toxicity; Cell Proliferation; Ethyl Methanesulfonate - toxicity; Gene Expression; Genome, Fungal; Haploinsufficiency; Mutagenesis; Mutagens - toxicity; Oxidants - toxicity; Oxidative Stress - genetics; Peroxidases - genetics; Phenotype; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae Proteins - genetics; Sequence Analysis; Sequence Analysis, DNA

The combination of functional genomics with next generation sequencing facilitates new experimental strategies for addressing complex biological phenomena. Here, we report the identification of a gain-of-function allele of peroxiredoxin (thioredoxin peroxidase, Tsa1p) via whole-genome re-sequencing...