Isolation and characterization of a mitochondrial d amino acid oxidase from Neurospora crassa

• Published in: Canadian journal of biochemistry Vol. 55; no. 1; pp. 66 - 74
• Main Authors: Rosenfeld, M.G, Leiter, E.H
• Format: Journal Article
• Language: English
• Published: Canada 1977
• Subjects: Cell Fractionation; D-Amino-Acid Oxidase - isolation & purification; D-Amino-Acid Oxidase - metabolism; fungi; Hydrogen-Ion Concentration; Kinetics; Mitochondria - enzymology; Neurospora - enzymology; Neurospora crassa - enzymology; Structure-Activity Relationship; Temperature

D-Amino acid oxidase (EC 1.4.3.3) activity in homogenates of Neurospora crassa strain SY7A was found to sediment with the mitochondrial fraction. Digitonin fractionation studies on purified mitochondria have indicated a matrix localization of the enzyme. Additionally, a peroxidase (EC 1.11.1.7) acti...