Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks
Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area...
Saved in:
Published in | Biologia plantarum Vol. 64; no. 1; pp. 32 - 42 |
---|---|
Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Institute of Experimental Botany of the Czech Academy of Sciences
01.01.2020
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes. |
---|---|
AbstractList | Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes. |
Author | SHI, Y. HAN, Y.Q. ZHENG, D.F. FENG, N.J. GAO, Y.M. ZHANG, Y.X. DU, J.D. |
Author_xml | – sequence: 1 givenname: Y.Q. surname: HAN fullname: HAN, Y.Q. – sequence: 2 givenname: Y. surname: SHI fullname: SHI, Y. – sequence: 3 givenname: Y.M. surname: GAO fullname: GAO, Y.M. – sequence: 4 givenname: J.D. surname: DU fullname: DU, J.D. – sequence: 5 givenname: N.J. surname: FENG fullname: FENG, N.J. – sequence: 6 givenname: Y.X. surname: ZHANG fullname: ZHANG, Y.X. – sequence: 7 givenname: D.F. surname: ZHENG fullname: ZHENG, D.F. |
BookMark | eNo9kMtO3DAUhq0KpA6XHQ_gByBT32InywqVi4TEBtbWsXM8mCZ2ZFui8_YMA-rqvyy-xXdGTlJOSMgVZ1spNO9_uXUrGB-3XKgfZMN7I7tBaHVCNowx3Uku1U9yVuvbYY6GqQ1JzwWhLZgazYHezXsfE9IF_tGKOFX6Htsr3UXnsOA8x1QpzA1LpSXnRpdc1tc8593-mkKClpdjmWgrkKovcW0xJ5hpwvaey996QU4DzBUvv_OcvNz-eb657x6f7h5ufj92Xoy6dX0IaFD5wU2DmpwcnddKKDe50RkOCKYXCpR3QWkJnrmBDYZrOUIwwcMoz8nDF3fK8GbXEhcoe5sh2uORy85CadHPaI1gTPVg2AROca4HjoMemewN81xgOLCuv1i-5FoLhv88zuzRu3Wr_fRuD97lB1_Zegg |
Cites_doi | 10.1105/tpc.011650 10.1093/aob/mcv160 10.1104/pp.16.01099 10.1186/gb-2013-14-4-r36 10.1093/bioinformatics/btq675 10.1016/S1016-8478(23)17584-4 10.1016/j.cub.2009.06.023 10.1007/s11103-008-9417-2 10.1094/MPMI-11-10-0271 10.1186/s12864-016-2378-y 10.1080/15592324.2015.1106659 10.1016/S1369-5266(02)00279-0 10.1186/s12870-015-0679-y 10.1105/tpc.005975 10.1105/tpc.109.073239 10.1007/s12374-017-0028-9 10.1186/s12864-015-1324-8 10.1038/nature01387 10.1046/j.1365-313X.2003.01973.x 10.1146/annurev.arplant.58.032806.104006 10.1111/tpj.12478 10.1104/pp.124.1.231 10.1093/genetics/162.3.1445 10.1007/s10535-018-0809-0 10.1016/j.cub.2009.05.059 10.1093/aob/mcs049 10.1038/77355 10.1105/tpc.106.043729 10.1111/pbi.12766 10.1016/j.bbrc.2015.04.109 10.1104/pp.010259 |
ContentType | Journal Article |
DBID | AAYXX CITATION DOA |
DOI | 10.32615/bp.2019.124 |
DatabaseName | CrossRef DOAJ Directory of Open Access Journals |
DatabaseTitle | CrossRef |
DatabaseTitleList | |
Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Botany |
EISSN | 1573-8264 |
EndPage | 42 |
ExternalDocumentID | oai_doaj_org_article_720045a70dab411681e86903570c12ef 10_32615_bp_2019_124 |
GroupedDBID | -4W -56 -5G -BR -~C .86 .VR 06C 06D 0VY 1N0 203 23N 29~ 2J2 2KG 2KM 2LR 2~H 30V 4.4 408 409 40D 40E 53G 5GY 5VS 67N 67Z 6NX 78A 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AAIAL AAJKR AARTL AATVU AAWCG AAYIU AAYQN AAYXX ABBBX ABBXA ABHLI ABJNI ABJOX ABKTR ABMNI ABNWP ABPLI ABQBU ABTHY ACGFS ACHXU ACKNC ACOMO ACPRK ACSNA ADHHG ADHIR ADINQ ADKPE ADRFC ADZKW AEGAL AEGNC AEJHL AENEX AEOHA AEPYU AETLH AEXYK AFBBN AFRAH AFWTZ AFZKB AGAYW AGQMX AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHSBF AHYZX AIIXL AJRNO AKMHD ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMYQR ARMRJ ASPBG AVWKF AZFZN B-. BA0 CITATION CS3 CSCUP DL5 DU5 ECGQY ESBYG FEDTE FNLPD FRP FRRFC FWDCC G-Y G-Z GGCAI GGRSB GQ6 GQ7 GQ8 GROUPED_DOAJ GXS HF~ HG5 HG6 HMJXF HQYDN HRMNR HVGLF HZ~ I09 IJ- ITM IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX KDC KOV KPH LAK MA- N9A NB0 NF0 O93 O9G O9I O9J OAM OK1 P19 PF0 QOK QOR QOS R89 R9I RNS RPX RSV S16 S27 S3A S3B SAP SBL SDH SDM SHX SISQX SJN SNE SNX SOJ SPISZ SSXJD SZN T13 TSG TSK TSV U2A U9L UG4 VC2 W23 W48 WJK WK8 YLTOR Z45 Z7V Z7W Z7Y Z8P Z8Q Z8S ZOVNA ~A9 ~EX EBS |
ID | FETCH-LOGICAL-c296t-5ffe7e4c8bd84db39bc6424bdb9b71aea7524a4cbf463ac0b80871639af7fca93 |
IEDL.DBID | DOA |
ISSN | 0006-3134 |
IngestDate | Tue Oct 22 15:09:02 EDT 2024 Fri Aug 23 04:34:35 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 1 |
Language | English |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c296t-5ffe7e4c8bd84db39bc6424bdb9b71aea7524a4cbf463ac0b80871639af7fca93 |
OpenAccessLink | https://doaj.org/article/720045a70dab411681e86903570c12ef |
PageCount | 11 |
ParticipantIDs | doaj_primary_oai_doaj_org_article_720045a70dab411681e86903570c12ef crossref_primary_10_32615_bp_2019_124 |
PublicationCentury | 2000 |
PublicationDate | 2020-01-01 |
PublicationDateYYYYMMDD | 2020-01-01 |
PublicationDate_xml | – month: 01 year: 2020 text: 2020-01-01 day: 01 |
PublicationDecade | 2020 |
PublicationTitle | Biologia plantarum |
PublicationYear | 2020 |
Publisher | Institute of Experimental Botany of the Czech Academy of Sciences |
Publisher_xml | – name: Institute of Experimental Botany of the Czech Academy of Sciences |
References | ref13 Farquharson (ref5) 1835 ref15 ref31 ref30 ref33 ref10 Rao (ref22) 2013; 3 ref32 ref0 Yuan (ref34) 1880 ref2 ref1 ref17 Li (ref14) 2009 ref16 ref19 ref18 ref24 Friedrichsen (ref6) 2002; 162 ref23 ref26 ref25 ref20 Fan (ref4) 2017; 16 ref21 ref28 ref27 ref29 ref8 ref7 Jung (ref11) 2008; 25 ref9 ref3 Katja (ref12) 2015; 10 |
References_xml | – ident: ref18 doi: 10.1105/tpc.011650 – start-page: 2017 year: 1880 ident: ref34 article-title: Jasmonate regulates plant responses to postsubmergence reoxygenation through transcriptional activation of antioxidant synthesis. - publication-title: Plant Physiol contributor: fullname: Yuan – ident: ref25 doi: 10.1093/aob/mcv160 – ident: ref28 doi: 10.1104/pp.16.01099 – ident: ref13 doi: 10.1186/gb-2013-14-4-r36 – ident: ref26 doi: 10.1093/bioinformatics/btq675 – volume: 25 start-page: 294 year: 2008 ident: ref11 article-title: Arabidopsis histidine-containing phosphotransfer factor 4 (AHP4) negatively regulates secondary wall thickening of the anther endothecium during flowering. - publication-title: Mol. Cells doi: 10.1016/S1016-8478(23)17584-4 contributor: fullname: Jung – ident: ref31 doi: 10.1016/j.cub.2009.06.023 – ident: ref8 doi: 10.1007/s11103-008-9417-2 – ident: ref21 doi: 10.1094/MPMI-11-10-0271 – ident: ref27 doi: 10.1186/s12864-016-2378-y – start-page: 2014 year: 1835 ident: ref5 article-title: SAUR19 links auxin and plasma membrane H+-ATPases in cell expansion. - publication-title: Plant Cell contributor: fullname: Farquharson – volume: 10 start-page: e1106659 year: 2015 ident: ref12 article-title: Dynamic subnuclear relocalization of WRKY40, a potential new mechanism of ABA-dependent transcription factor regulation. - publication-title: Plant Signal Behav doi: 10.1080/15592324.2015.1106659 contributor: fullname: Katja – ident: ref20 doi: 10.1016/S1369-5266(02)00279-0 – ident: ref33 doi: 10.1186/s12870-015-0679-y – ident: ref24 doi: 10.1105/tpc.005975 – ident: ref9 doi: 10.1105/tpc.109.073239 – ident: ref10 doi: 10.1007/s12374-017-0028-9 – ident: ref2 doi: 10.1186/s12864-015-1324-8 – ident: ref7 doi: 10.1038/nature01387 – ident: ref29 doi: 10.1046/j.1365-313X.2003.01973.x – volume-title: Botanical Microtechnique year: 2009 ident: ref14 contributor: fullname: Li – ident: ref19 doi: 10.1146/annurev.arplant.58.032806.104006 – ident: ref15 doi: 10.1111/tpj.12478 – ident: ref23 doi: 10.1104/pp.124.1.231 – volume: 162 start-page: 1445 year: 2002 ident: ref6 article-title: Three redundant brassinosteroid early response genes encode putative bHLH transcription factors required for normal growth publication-title: Genetics doi: 10.1093/genetics/162.3.1445 contributor: fullname: Friedrichsen – ident: ref16 doi: 10.1007/s10535-018-0809-0 – ident: ref0 doi: 10.1016/j.cub.2009.05.059 – ident: ref30 doi: 10.1093/aob/mcs049 – ident: ref3 doi: 10.1038/77355 – ident: ref32 doi: 10.1105/tpc.106.043729 – volume: 16 start-page: 254 year: 2017 ident: ref4 article-title: Ectopic expression of a novel OsExtensin-like gene consistently enhances plant lodging resistance by regulating cell elongation and cell wall thickening in rice. - publication-title: Plant Biotechnol doi: 10.1111/pbi.12766 contributor: fullname: Fan – volume: 3 start-page: 71 year: 2013 ident: ref22 article-title: An improvement of the 2-∆∆Ct method for quantitative real-time polymerase chain reaction data analysis publication-title: Biostat. Bioinform. Biomath contributor: fullname: Rao – ident: ref17 doi: 10.1016/j.bbrc.2015.04.109 – ident: ref1 doi: 10.1104/pp.010259 |
SSID | ssj0009704 |
Score | 2.2892294 |
Snippet | Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood.... |
SourceID | doaj crossref |
SourceType | Open Website Aggregation Database |
StartPage | 32 |
SubjectTerms | gene expression lateral root main root rna-seq seed soaking soybean |
Title | Treatment of Glycine max seeds with gibberellins alters root morphology, anatomy, and transcriptional networks |
URI | https://doaj.org/article/720045a70dab411681e86903570c12ef |
Volume | 64 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3PS8MwFA4iHryIP3H-Ige9Wde0SdMcnTiHoKcNdit5aSKC68Zawf33vqSrzJMXb6WUtnwv5H3fy8sXQq5jrbxboYogEzziJU8jHMomMr7coJ3fju7rHS-v2WjCn6diunHUl-8Ja-2BW-D60odRaBmXGjhjWc6sP0QpFTI2LLEuzL5MdGKqs9uVMW_b3JGeMNEHb03J1B1L-K8EtOHTHxLKcJ_srZkgvW__4IBs2eqQ7AzmyNZWR6Qadx3gdO7o08fKL4DTmf6iNeabmvr6KX17B7BLb6lZ1TSse9cUmXBDZ3OELxTMb6muUFfPwkVJG5-auokCv161XeD1MZkMH8cPo2h9NkJkEpU1kXDOSstNDmXOS0gVGFQSHEpQIJm2WoqEa27A8SzVJgaMCEqjVGknndEqPSHb1byyp4QmKbBUc6sMkivkT9o6CTaOITaC2TLvkZsOsGLRWmAUKB0CsAUsCg9sgcD2yMCj-fOMN64ONzCcxTqcxV_hPPuPl5yT3cTL4lApuSDbzfLTXiJ3aOAqDJNvoMDCew |
link.rule.ids | 315,786,790,870,2115,27955,27956 |
linkProvider | Directory of Open Access Journals |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Treatment+of+Glycine+max+seeds+with+gibberellins+alters+root+morphology%2C+anatomy%2C+and+transcriptional+networks&rft.jtitle=Biologia+plantarum&rft.au=Y.Q.+HAN&rft.au=Y.+SHI&rft.au=Y.M.+GAO&rft.au=J.D.+DU&rft.date=2020-01-01&rft.pub=Institute+of+Experimental+Botany+of+the+Czech+Academy+of+Sciences&rft.eissn=1573-8264&rft.volume=64&rft.issue=1&rft.spage=32&rft.epage=42&rft_id=info:doi/10.32615%2Fbp.2019.124&rft.externalDBID=DOA&rft.externalDocID=oai_doaj_org_article_720045a70dab411681e86903570c12ef |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3134&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3134&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3134&client=summon |