Label-free 3D tracking of biological micro-objects using RGB pinholes

Abstract Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a volume through image analysis. For three-dimensional (3D) tracking, approaches utilizing defocusing techniques have been proposed to enable depthw...

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Published inJapanese Journal of Applied Physics Vol. 63; no. 4; pp. 4 - 12
Main Authors Kim, Doyeop, Matsuzaki, Takahisa, Yoshikawa, Hiroshi Y., Kawamura, Ryuzo
Format Journal Article
LanguageEnglish
Published Tokyo IOP Publishing 01.04.2024
Japanese Journal of Applied Physics
Subjects
3D-tracking
cell
Color coding
Defocusing
Digital imaging
Digital particle image velocimetry
Fluidics
Image analysis
Labels
microscopy
Microtechnology
Molecular motors
Particle image velocimetry
particles
Pinholes
Three dimensional analysis
Tracking
trajectory
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Abstract Abstract Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a volume through image analysis. For three-dimensional (3D) tracking, approaches utilizing defocusing techniques have been proposed to enable depthwise positional measurements. Recently, there have been suggestions to simplify this approach by employing a triple pinhole mask and to further enhance it with color filters that allow distinguish particles, even under dense conditions. This paper presents an attempt to apply the defocusing method using a triple pinhole mask with red, green, and blue (RGB) color-coding filters, demonstrating the successful label-free 3D tracking of biological micro-objects at multiple positions within a volume. The application of this method in fluidics and its potential for biological analysis are highlighted through successful examples of 3D tracking involving micro-particles influenced by motor proteins, dividing cells, and swimming microorganisms.
AbstractList Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a volume through image analysis. For three-dimensional (3D) tracking, approaches utilizing defocusing techniques have been proposed to enable depthwise positional measurements. Recently, there have been suggestions to simplify this approach by employing a triple pinhole mask and to further enhance it with color filters that allow distinguish particles, even under dense conditions. This paper presents an attempt to apply the defocusing method using a triple pinhole mask with red, green, and blue (RGB) color-coding filters, demonstrating the successful label-free 3D tracking of biological micro-objects at multiple positions within a volume. The application of this method in fluidics and its potential for biological analysis are highlighted through successful examples of 3D tracking involving micro-particles influenced by motor proteins, dividing cells, and swimming microorganisms.
Abstract Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a volume through image analysis. For three-dimensional (3D) tracking, approaches utilizing defocusing techniques have been proposed to enable depthwise positional measurements. Recently, there have been suggestions to simplify this approach by employing a triple pinhole mask and to further enhance it with color filters that allow distinguish particles, even under dense conditions. This paper presents an attempt to apply the defocusing method using a triple pinhole mask with red, green, and blue (RGB) color-coding filters, demonstrating the successful label-free 3D tracking of biological micro-objects at multiple positions within a volume. The application of this method in fluidics and its potential for biological analysis are highlighted through successful examples of 3D tracking involving micro-particles influenced by motor proteins, dividing cells, and swimming microorganisms.
Author Matsuzaki, Takahisa
Yoshikawa, Hiroshi Y.
Kim, Doyeop
Kawamura, Ryuzo
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Snippet Abstract Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a...
Defocusing digital particle image velocimetry is a method developed within the field of fluidics to measure the velocities of particles within a volume through...
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SubjectTerms 3D-tracking
cell
Color coding
Defocusing
Digital imaging
Digital particle image velocimetry
Fluidics
Image analysis
Labels
microscopy
Microtechnology
Molecular motors
Particle image velocimetry
particles
Pinholes
Three dimensional analysis
Tracking
trajectory
Title Label-free 3D tracking of biological micro-objects using RGB pinholes
URI https://iopscience.iop.org/article/10.35848/1347-4065/ad3969
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