Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum

• Published in: Analyst (London) Vol. 15; no. 2; pp. 395 - 44
• Main Authors: Sheng, Xiuqin, Chen, Jiakai, Shao, Jiahui, Zhang, Xiaoya, Wang, Bing, Ding, Chuan-Fan, Yan, Yinghua
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 13.01.2025
• Subjects: Acrylamides - chemistry; Blood coagulation; Cancer; Colorectal cancer; Colorectal Neoplasms - blood; Epoxy Compounds - chemistry; Glycopeptides; Glycoproteins; Glycoproteins - analysis; Heparin; Humans; Hydrophilicity; Hydrophobic and Hydrophilic Interactions; Methylene bisacrylamide; Pathogenesis; Phosphoproteins - analysis; Phosphorylation; Proteomics - methods; Recovery; Recyclability; Ring opening; Sensitivity; Serum - chemistry; Titanium; Titanium - chemistry; Vinyl Compounds - chemistry

The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N , N -methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic group...