Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum
The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N , N -methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic group...
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| Published in | Analyst (London) Vol. 15; no. 2; pp. 395 - 44 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
Royal Society of Chemistry
13.01.2025
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and
N
,
N
-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (
Cys-PVME-Ti
4+
) for the enrichment of phosphopeptides and glycopeptides.
Cys-PVME-Ti
4+
has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g
−1
, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL
−1
), selectivity (1 : 2000), a loading capacity of 62.5 mg g
−1
, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.
This work introduces a titanium-functionalized polymeric material rich in hydrophilic groups for phosphopeptide and glycopeptide enrichment in serum. |
|---|---|
| AbstractList | The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and
N
,
N
-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (
Cys-PVME-Ti
4+
) for the enrichment of phosphopeptides and glycopeptides.
Cys-PVME-Ti
4+
has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g
−1
, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL
−1
), selectivity (1 : 2000), a loading capacity of 62.5 mg g
−1
, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.
This work introduces a titanium-functionalized polymeric material rich in hydrophilic groups for phosphopeptide and glycopeptide enrichment in serum. The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g−1, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL−1), selectivity (1 : 2000), a loading capacity of 62.5 mg g−1, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis. The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g-1, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL-1), selectivity (1 : 2000), a loading capacity of 62.5 mg g-1, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis.The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N,N-methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g-1, recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL-1), selectivity (1 : 2000), a loading capacity of 62.5 mg g-1, recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis. The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and , -methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g , recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL ), selectivity (1 : 2000), a loading capacity of 62.5 mg g , recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis. The analysis of protein phosphorylation and glycosylation is critical for investigating disease development. In this work, 1,2-epoxy-5-hexene and N , N -methylenebisacrylamide were polymerized with vinyl phosphate to produce a polymer (denoted as PVME), which contained a variety of hydrophilic groups. The material's hydrophilicity was further enhanced by a ring-opening reaction with cysteine (the product was denoted as Cys-PVEM). Finally, titanium sulfate was combined with Cys-PVME to form titanium-rich polymers (Cys-PVME-Ti4+) for the enrichment of phosphopeptides and glycopeptides. Cys-PVME-Ti4+ has a good sensitivity (0.02 fmol) and selectivity (1 : 1000) with a loading capacity of 62 mg g −1 , recyclability (9 cycles), and a good recovery rate (101.6 ± 0.60%) for phosphopeptides, and good sensitivity (0.01 fmol μL −1 ), selectivity (1 : 2000), a loading capacity of 62.5 mg g −1 , recyclability (9 cycles), and a good recovery rate (98.7 ± 1.2%) for glycopeptides. In addition, after enrichment with this material, 27 phosphopeptides with 14 phosphoproteins and 223 glycopeptides associated with 88 glycoproteins were captured from the serum of colorectal cancer patients, while 27 phosphopeptides associated with 14 phosphoproteins and 210 glycopeptides associated with 111 glycoproteins were also captured from the serum of a normal control. Gene ontology (GO) analysis revealed that complement activation, extracellular region, extracellular space, blood coagulation, the IgG immunoglobulin complex, and heparin binding were different between normal control and colorectal cancer, implying that related pathways are likely involved in colorectal cancer pathogenesis. |
| Author | Zhang, Xiaoya Sheng, Xiuqin Yan, Yinghua Ding, Chuan-Fan Shao, Jiahui Wang, Bing Chen, Jiakai |
| AuthorAffiliation | Ningbo University Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province School of Material Science and Chemical Engineering Institute of Mass Spectrometry |
| AuthorAffiliation_xml | – name: Ningbo University – name: School of Material Science and Chemical Engineering – name: Institute of Mass Spectrometry – name: Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province |
| Author_xml | – sequence: 1 givenname: Xiuqin surname: Sheng fullname: Sheng, Xiuqin – sequence: 2 givenname: Jiakai surname: Chen fullname: Chen, Jiakai – sequence: 3 givenname: Jiahui surname: Shao fullname: Shao, Jiahui – sequence: 4 givenname: Xiaoya surname: Zhang fullname: Zhang, Xiaoya – sequence: 5 givenname: Bing surname: Wang fullname: Wang, Bing – sequence: 6 givenname: Chuan-Fan surname: Ding fullname: Ding, Chuan-Fan – sequence: 7 givenname: Yinghua surname: Yan fullname: Yan, Yinghua |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/39704554$$D View this record in MEDLINE/PubMed |
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| Cites_doi | 10.1016/j.microc.2022.107397 10.1016/j.cclet.2022.03.075 10.1016/j.bbrc.2022.07.051 10.1016/j.chroma.2022.462929 10.1016/j.cclet.2022.07.059 10.1016/j.talanta.2022.123956 10.1007/s00604-022-05343-0 10.2147/IJGM.S380208 10.3389/fimmu.2022.810993 10.1016/j.cclet.2021.12.069 10.1158/0008-5472.CAN-20-1541 10.3390/cancers14061543 10.3390/jcm12227173 10.1039/d3an00709j 10.1039/d2ra04609a 10.1007/s00604-022-05394-3 10.1007/s00604-023-05952-3 10.1002/jssc.202100993 10.1039/d1an01182k 10.1007/s00604-023-06104-3 10.1016/j.talanta.2023.124771 10.1007/s00604-020-04652-6 10.1093/ofid/ofad547 10.1007/s00216-023-04575-0 10.1016/j.foodchem.2020.127944 10.1007/s00604-022-05500-5 10.3938/jkps.69.578 10.1016/j.chroma.2022.463599 10.3389/fonc.2023.1143122 10.1016/j.chroma.2023.463880 10.1016/j.micromeso.2022.112066 10.1007/s00216-022-04323-w 10.1021/am900301x 10.1016/S0304-3835(98)00275-4 10.1002/jssc.200600199 10.1016/j.microc.2023.109590 10.1016/j.cclet.2022.107829 10.1021/acssuschemeng.1c02119 10.1016/j.trac.2023.117234 10.1039/d3an01473h 10.1016/j.aca.2021.338412 10.1016/j.chroma.2021.462072 10.1093/brain/awaa373 10.1007/s00216-023-04857-7 10.1186/s13041-021-00852-0 10.3390/nano7090272 10.1016/j.chroma.2022.463406 10.1016/j.gca.2009.06.031 10.1039/d3fo03125j 10.1039/d2an02004a 10.1016/j.trac.2020.116168 10.1039/d1gc03290a 10.1016/j.trac.2022.116881 |
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| SubjectTerms | Acrylamides - chemistry Blood coagulation Cancer Colorectal cancer Colorectal Neoplasms - blood Epoxy Compounds - chemistry Glycopeptides Glycoproteins Glycoproteins - analysis Heparin Humans Hydrophilicity Hydrophobic and Hydrophilic Interactions Methylene bisacrylamide Pathogenesis Phosphoproteins - analysis Phosphorylation Proteomics - methods Recovery Recyclability Ring opening Sensitivity Serum - chemistry Titanium Titanium - chemistry Vinyl Compounds - chemistry |
| Title | Preparation of a titanium-functionalized polymeric material rich in hydrophilic groups for phosphoproteome and glycoproteome analyses in serum |
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