Effect of ouabain and bumetanide on the basal and the osmolality-affected prolactin secretion from human decidual cells in vitro

• Published in: European journal of obstetrics & gynecology and reproductive biology Vol. 23; no. 3; pp. 159 - 166
• Main Authors: Andersen, Jesper Rye, Borggaard, Birgit, Olsen, Elith Bjarne, Stimpel, Hans, Nyholm, Henrik Christian, Schroeder, Erik
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.11.1986; Elsevier
• Subjects: Biological and medical sciences; Bumetanide; Bumetanide - pharmacology; Decidua - drug effects; Decidua - metabolism; Decidual prolactin secretion; Diuretics - pharmacology; Female; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Humans; Hyperosmotic medium; In Vitro Techniques; Mannitol - pharmacology; Osmolality; Osmolar Concentration; Ouabain; Ouabain - pharmacology; Potassium Chloride - pharmacology; Pregnancy; Pregnancy. Parturition. Lactation; Prolactin - metabolism; Radioimmunoassay; Sodium Chloride - pharmacology; Vertebrates: reproduction; Hyperosmotic medium; Bumetanide; Decidual prolactin secretion; Osmolality; Ouabain; Pregnancy; Human; Protein hormone; Prolactin; Gonadotropin; Decidua; Osmotic pressure; In vitro
• ISSN: 0301-2115; 1872-7654
• DOI: 10.1016/0028-2243(86)90144-9

Human decidual explants were incubated in vitro in media containing 10 −5 mol/l ouabain or 10 −4 mol/l ouabain or 0.5 · 10 −4 mol/l bumetanide to change the intracellular ionic concentrations. In isosmotic incubations (osmolality 315 mmol/kg), no significant effect of either 10 −5 mol/l ouabain or 0.5 · 10 −4 mol/l bumetanide was found on the decidual prolactin secretion (D-PRL-s). 10 −4 mol/l ouabain significantly decreased both decidual prolactin production and D-PRL-s ( P < 0.05) and was therefore not used for the following cross-over experiments. Hyperosmotic media (387 mmol/kg) were produced by changing the concentration of either sodium chloride, potassium chloride, or mannitol. All increased D-PRL-s compared with the isosmotic media (315 mmol/kg). 10 −5 mol/l ouabain significantly diminished the increase otherwise elicited by the sodium chloride and the mannitol hyperosmotic media. However, in the hyperosmotic potassium chloride medium with 10 −5 mol/l ouabain, the D-PRL-s remained increased. The hyperosmotic medium (252 mmol/kg) reduced D-PRL-s compared with the isosmotic media (315 mmol/kg) and no significant effect of ouabain was found. Bumetanide did not change the D-PRL-s into any of the hypo- or hyperosmotic media compared with the secretion at 315 mmol/kg. Based on experience from other cell types, the results further indicate that the intracellular ionic concentrations could be of importance to the secretion of decidual prolactin in vitro.