Brevibacillus ruminantium sp. nov., isolated from cow faeces

• Published in: International journal of systematic and evolutionary microbiology Vol. 73; no. 12
• Main Authors: Kim, Seunghwan, Heo, Jun, Kwon, Soon-Wo, Lee, Daseul, Han, Byeong-Hak, Hong, Seung-Beom, Kim, Yiseul
• Format: Journal Article
• Language: English
• Published: England 01.12.2023
• Subjects: Animals; Bacterial Typing Techniques; Base Composition; Brevibacillus; Cattle; DNA, Bacterial - genetics; Fatty Acids - chemistry; Phospholipids; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sodium Chloride; Brevibacillus ruminantium sp. nov; taxonomy; novel species; cow faeces
• DOI: 10.1099/ijsem.0.006204

An aerobic, Gram-stain-positive, rod-shaped, endospore-forming bacterial strain, designated BB3-R1 , was isolated from cow faeces sampled in Daejeon, Republic of Korea. Growth was observed at 25-45 °C (optimum, 35-40 °C) and pH 7.0-9.0 (optimum, pH 8.0), with up to 3 % (w/v) NaCl (optimum, 0 % NaCl). blast analysis of 16S rRNA gene sequences revealed the highest sequence similarity of strain BB3-R1 to NRRL NRS-818 (98.8 %) followed by JCM 15085 (97.5 %). According to 16S rRNA gene and whole-genome based phylogenetic trees, strain BB3-R1 clustered with FJAT-54423 and NRRL NRS-818 . OrthoANI and dDDH values of strain BB3-R1 with the closely related strains were lower than 77.5 and 26.8 %, respectively. The major menaquinones and polar lipids of the strain were MK-7 and phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, respectively. The major fatty acids (>10 %) were C iso, C iso, C anteiso and C   7 alcohol. The cell-wall peptidoglycan contained cross-linked -diaminopimelic acid (type A1 gamma). The phenotypic, chemotaxonomic and genotypic data obtained in this study showed that the strain represents a novel species of the genus , for which the name sp. nov. (type strain BB3-R1 =KACC 22663 =NBRC 115962 ) is proposed.