Adenovirus-mediated expression of both antisense ODC and-AdoMetDC inhibited colorectal cancer cell growth in vitro

• Published in: Acta pharmacologica Sinica Vol. 27; no. 3; pp. 353 - 359
• Main Authors: Zhang, Bing, Liu, Xian-xi, Zhang, Yan, Jiang, Chun-ying, Teng, Qing-shan, Hu, Hai-yan, Wang, Wei, Gong, Lei
• Format: Journal Article
• Language: English
• Published: United States Nature Publishing Group 01.03.2006
• Subjects: Adenosylmethionine Decarboxylase - genetics; Adenosylmethionine Decarboxylase - metabolism; Adenoviridae - genetics; Adenovirus; Cell Line; Cell Proliferation; Embryo, Mammalian; Genetic Vectors; HT29 Cells - metabolism; HT29 Cells - pathology; Humans; Kidney - cytology; Oligodeoxyribonucleotides, Antisense; Ornithine Decarboxylase - genetics; Ornithine Decarboxylase - metabolism; Polyamines - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Transfection; 直肠癌; 细胞生长因子; 结肠癌; 肿瘤细胞; 腺病毒

Aim： To construct a recombinant adenovirus that can simultaneously express both antisense oruithine decarboxylase （ODC） and adenosylmethionine decarboxylase （ AdoMetDC ） and detect its inhibitory effect on the intracellular polyamine pool and colorectal cancer cell growth. Methods： A 205-bp cDNA of...