In vitro development of ovine embryos in CZB medium
One- to four-cell embryos were collected from multiparous crossbred ewes and were cultured in vitro for 120 hours in CZB medium. A 2×2 factorial treatment arrangement was used to examine the effects of glucose and ethylenediaminetetraacetic acid (EDTA) on in vitro embryo development. The embryos wer...
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Published in | Theriogenology Vol. 37; no. 3; pp. 559 - 569 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
01.03.1992
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Subjects | |
Online Access | Get full text |
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Summary: | One- to four-cell embryos were collected from multiparous crossbred ewes and were cultured in vitro for 120 hours in CZB medium. A 2×2 factorial treatment arrangement was used to examine the effects of glucose and ethylenediaminetetraacetic acid (EDTA) on in vitro embryo development. The embryos were examined every 12 hours, and all of the embryos were stained with a DNA-specific fluorochrome after the 120-hour evaluation to enable the counting of cell nuclei. Embryo development was analyzed for cleavage beyond 16 cells as well as for cleavage to at least the compact morula stage based upon both the 120-hour morphological evaluation and nuclear counts. Forty-eight percent of the embryos passed through the in vitro developmental block (i.e., cleaved beyond 16 cells), and 26% developed to 30 or more cells. Neither EDTA nor glucose affected in vitro embryo development based on the nuclear counts. |
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Bibliography: | L70 9196016 L53 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0093-691X 1879-3231 |
DOI: | 10.1016/0093-691X(92)90136-F |