Preparation and properties of poly(ethylene glycol)-b-poly(3-acrylamidophenylboronic acid) glucose responsive particles and their hyaluronic acid-based microneedle patches

• Published in: Journal of Zhejiang University. A. Science Vol. 25; no. 5; pp. 395 - 410
• Main Authors: Hong, Yichuan, Yu, Haojie, Wang, Li, Chen, Xiang, Shen, Di, Wang, Yu, Ren, Shuning, Huang, Yudi, Yang, Jian
• Format: Journal Article
• Language: English
• Published: Hangzhou Zhejiang University Press 01.05.2024; Springer Nature B.V; State Key Laboratory of Chemical Engineering,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310058,China%State Key Laboratory of Chemical Engineering,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310058,China; Zhejiang-Russia Joint Laboratory of Photo-Electro-Magnetic Functional Materials,College of Chemical and Biological Engineering,Zhejiang University,Hangzhou 310058,China
• Subjects: Biosafety; Block copolymers; Blood; Blood glucose; Blood levels; Chain transfer; Civil Engineering; Classical and Continuum Physics; Engineering; Glucose; Hyaluronic acid; Hydrophobicity; Hypoglycemia; Industrial Chemistry/Chemical Engineering; Insulin; Mechanical Engineering; Needles; Polyethylene glycol; Research Article; Self-assembly; Substrates; Synthesis; 苯硼酸; 透明质酸; Microneedles; Phenylboronic acid (PBA); 微针; Diabetes; Glucose responsive hydrogel; Hyaluronic acid; 糖尿病; 糖敏水凝胶; Phenylboronic acid(PBA)
• DOI: 10.1631/jzus.A2300386
• ISSN: 1673-565X

To overcome the pain and risk of hypoglycemia in insulin administration, glucose-responsive microneedles have been developed by researchers, which could release insulin according to the blood glucose level. We designed a kind of particles by a reversible addition-fragmentation chain transfer (RAFT) method containing a phenylboronic acid group as the sensor of glucose and carrier of insulin. poly(ethylene glycol) (PEG)-2-(dodecylthio(thiocarbonyl)thio)-2-methylpropionic acid (DDMAT) was synthesized as a macromolecular RAFT agent, which was then reacted with 3-acrylamidophenylboronic acid (AAPBA) to synthesize the block copolymer PEG- b -PAAPBA. Glucose-responsive particles loaded with insulin were prepared by self-assembly based on hydrophilic-hydrophobic interactions. Microneedle patches loaded with glucose-responsive particles were prepared using hyaluronic acid as the substrate. The insulin release behavior of the particles in glucose solutions of 0, 100, and 400 mg/dL showed significant glucose responsiveness and good biosafety. The results of blood glucose control experiments in rats indicate that a single microneedle patch can effectively maintain normal blood glucose for over 7 h.