A glycoprotein from neurites of differentiated neuroblastoma cells

Neurites were prepared by a novel method from differentiating mouse neuroblastoma cells. When electrically differentiated cells were labeled metabolically with L-[3H]fucose or D-[3H]glucosamine, both the neurites and the surface membranes showed the presence of a glycoprotein of apparent Mr = 200,00...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of biological chemistry Vol. 255; no. 11; pp. 5448 - 5453
Main Authors Littauer, U Z, Giovanni, M Y, Glick, M C
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 10.06.1980
Subjects
Animals
Cell Differentiation
Cell Line
Glycoproteins - analysis
Membrane Proteins - analysis
Mice
Molecular Weight
Neuroblastoma - analysis
Online AccessGet full text

Cover

More Information
Summary:Neurites were prepared by a novel method from differentiating mouse neuroblastoma cells. When electrically differentiated cells were labeled metabolically with L-[3H]fucose or D-[3H]glucosamine, both the neurites and the surface membranes showed the presence of a glycoprotein of apparent Mr = 200,000. In contrast, the level of this glycoprotein was reduced in the surface membranes from nondifferentiated cells and a radioactive glycoprotein of similar molecular weight was found in the growth medium. The method for the isolation of neurites is of potential usage in distinguishing specific proteins associated with growing neurites.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(19)70807-2