Protein Synthesis via in vitro Transcription and Translation System inside Monodisperse Vesicles Fabricated by Microfluidics

• Published in: Electrical engineering in Japan Vol. 218; no. 2
• Main Authors: Nanjo, Satoshi, Tsugane, Mamiko, Matsuura, Tomoaki, Suzuki, Hiroaki
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc 01.06.2025
• Subjects: Bioreactors; Biosensors; Fluorescence; GUVs; IVTT system; liposome; membrane protein; Microfluidics; protein; Protein synthesis; Proteins; Vesicles

ABSTRACT In this study, we investigated the conditions for protein synthesis by an in vitro transcription and translation (IVTT) system within giant unilamellar vesicles (GUVs) produced with a microfluidic channel. The commercial IVTT system consisted of purified components (PURE system), and DNA en...