Role of hepatocyte growth factor in promoting the growth of human corneal endothelial cells stimulated by L-ascorbic acid 2-phosphate

• Published in: Investigative ophthalmology & visual science Vol. 53; no. 12; pp. 7583 - 7589
• Main Authors: Kimoto, Miwa, Shima, Nobuyuki, Yamaguchi, Masahiro, Amano, Shiro, Yamagami, Satoru
• Format: Journal Article
• Language: English
• Published: United States 09.11.2012
• Subjects: Antineoplastic Agents - pharmacology; Ascorbic Acid - analogs & derivatives; Ascorbic Acid - pharmacology; Blotting, Western; Cell Proliferation - drug effects; Cells, Cultured; Endothelium, Corneal - drug effects; Endothelium, Corneal - growth & development; Endothelium, Corneal - metabolism; Enzyme-Linked Immunosorbent Assay; Gene Expression Regulation, Developmental - drug effects; Hepatocyte Growth Factor - biosynthesis; Hepatocyte Growth Factor - genetics; Humans; RNA - genetics; Tissue Array Analysis

To investigate the mechanisms by which L-ascorbic acid 2-phosphate (Asc-2P) increases the proliferation of human corneal endothelial cells (HCECs). Growth of cultured HCECs was examined in the presence of various antioxidants, including Asc-2P, retinyl acetate (vitamin A), reduced glutathione, oxidi...