Negative-ion mass spectrometry of substituted adenine bases and adenosine nucleosides

• Published in: Rapid communications in mass spectrometry Vol. 3; no. 8; p. 249
• Main Authors: Hocart, C H, Schlunegger, U P
• Format: Journal Article
• Language: English
• Published: England 01.08.1989
• Subjects: Adenine - analogs & derivatives; Adenine - chemistry; Adenosine - analogs & derivatives; Adenosine - chemistry; Mass Spectrometry - methods
• ISSN: 0951-4198
• DOI: 10.1002/rcm.1290030802

The negative-ion chemical ionization (ammonia, 5 Pa source pressure) mass spectra of a series of substituted adenine bases, adenosine nucleosides, and the trimethylsilyl derivatives of the nucleosides are described. Selected ions from these spectra were subject to collisionally activated dissociation with mass-analysed ion kinetic energy (CAD/MIKE) analysis of the products and the spectra assessed for information content. In addition to observing strong peaks due to quasimolecular ions and heterocyclic-base ions, it proved possible to differentiate between 2'-, 3'- and 5'-deoxy and between 2'- and 3'-O-methyl isomers. The negative-ion chemical ionization spectra of four methyladenines are essentially identical, but could be clearly distinguished from each other by CAD/MIKE analysis.