Enabling rapid identification of biotherapeutic protein products using handheld Raman spectrometers and principal component analysis

• Published in: Journal of Raman spectroscopy Vol. 52; no. 7; pp. 1281 - 1293
• Main Authors: Soto, Robert J., Meriage, David S., Wagner, Robin L., Wang, Ting, Semin, David J.
• Format: Journal Article
• Language: English
• Published: Bognor Regis Wiley Subscription Services, Inc 01.07.2021
• Subjects: Algorithms; Analyzers; Biopharmaceuticals; Chemical compounds; identity; Methods; multivariate; Pharmacology; Preventive maintenance; Principal components analysis; Product differentiation; Proteins; Raman; Raman spectra; Raman spectroscopy; Spectrometers; Spectrum analysis; Wavelengths

Herein, the utility of Raman spectroscopy for rapid identity (ID) verification of biotherapeutic protein products in solution is demonstrated. The success of this approach hinges on the unique combination of Raman features associated with both the therapeutic agent and excipients as the basis for pr...