A method for evaluating the effects of ligands upon Gs protein-coupled receptors using a recombinant melanophore-based bioassay

• Published in: Analytical biochemistry Vol. 206; no. 2; pp. 315 - 322
• Main Authors: POTENZA, M. N, GRAMINSKI, G. F, LERNER, M. R
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier 01.11.1992
• Subjects: Animals; Biological and medical sciences; Biological Assay - methods; Cell receptors; Cell structures and functions; Cells, Cultured; Cyclic AMP - metabolism; Fundamental and applied biological sciences. Psychology; GTP-Binding Proteins - metabolism; Kinetics; Ligands; Melanocyte-Stimulating Hormones - pharmacology; Melanophores - cytology; Melanophores - metabolism; Metaproterenol - pharmacology; Molecular and cellular biology; Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine); Plasmids; Receptors, Adrenergic, beta - drug effects; Receptors, Adrenergic, beta - metabolism; Receptors, Adrenergic, beta - radiation effects; Recombinant Proteins - drug effects; Recombinant Proteins - metabolism; Transfection; Xenopus laevis; Human; Bioassay; Ligand; Xenopus laevis; Amphibia; β2-Adrenergic receptor; Salientia; Gene expression; Plasmid; Vertebrata; Binding capacity; Transfection; Investigation method; Melanophore

As an increasing number of medically important receptors that couple to stimulatory guanine nucleotide (Gs) proteins are isolated and cloned, there is an equally escalating need for methods to rapidly and reproducibly evaluate potential ligands for their properties as agonists or antagonists. Recent...