Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin αIIbβ3

• Published in: The Journal of cell biology Vol. 181; no. 7; pp. 1211 - 1222
• Main Authors: Watanabe, Naohide, Bodin, Laurent, Pandey, Manjula, Krause, Matthias, Coughlin, Shaun, Boussiotis, Vassiliki A, Ginsberg, Mark H, Shattil, Sanford J
• Format: Journal Article
• Language: English
• Published: The Rockefeller University Press 30.06.2008; Rockefeller University Press
• Subjects: Agonists; Antibodies; Cells; CHO cells; Cytometry; Fluorescence; Integrins; Megakaryocytes; Platelets; Stem cells

Platelet aggregation requires agonist-induced αIIbβ3 activation, a process mediated by Rap1 and talin. To study mechanisms, we engineered αIIbβ3 Chinese hamster ovary (CHO) cells to conditionally express talin and protease-activated receptor (PAR) thrombin receptors. Human PAR1 or murine PAR4 stimul...