Cryptosporidium oocysts in water for human consumption. Comparison of staining methods

A study was carried out on the presence of oocysts of Cryptosporidium spp. in water for human consumption in the Province of Salamanca, Spain, using four different staining techniques: Ziehl-Neelsen, auramine, indirect immunofluorescence with monoclonal antibodies (Meridian, USA) and direct immunofl...

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Bibliographic Details
Published inEuropean journal of epidemiology Vol. 10; no. 2; p. 215
Main Authors Rodríguez-Hernandez, J, Canut-Blasco, A, Ledesma-Garcia, M, Martín-Sánchez, A M
Format Journal Article
LanguageEnglish
Published Netherlands 01.04.1994
Subjects
Animals
Antibodies, Monoclonal
Benzophenoneidum
Coloring Agents
Cryptosporidium - parasitology
Evaluation Studies as Topic
Fluorescent Antibody Technique
Humans
Sensitivity and Specificity
Spain
Water - parasitology
Water Purification - methods
Water Supply
Spain
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Summary:A study was carried out on the presence of oocysts of Cryptosporidium spp. in water for human consumption in the Province of Salamanca, Spain, using four different staining techniques: Ziehl-Neelsen, auramine, indirect immunofluorescence with monoclonal antibodies (Meridian, USA) and direct immunofluorescence with monoclonal antibodies (Pasteur, France). The presence of oocysts was detected in the water used for the washing of sand filters in the four water purifying plants and in the drinking water of two of the six geographical areas studied. The Ziehl-Neelsen and auramine staining techniques were found to have excellent diagnostic correlation. Immunofluorescence stainings with monoclonal antibodies proved to be less sensitive.
ISSN:0393-2990
1573-7284
DOI:10.1007/BF01730373