Mycobacterium anyangense sp. nov., a rapidly growing species isolated from blood of Korean native cattle, Hanwoo (Bos taurus coreanae)

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. Pt_7; pp. 2277 - 2285
• Main Authors: Kim, Byoung-Jun, Kim, Jae-Myung, Kim, Bo-Ram, Lee, So-Young, Kim, GaNa, Jang, Yun-Ho, Ryoo, Soyoon, Jeon, Che-Ok, Jin, Hyun-Mi, Jeong, Joseph, Lee, Seon Ho, Lim, Ji-Hun, Kook, Yoon-Hoh, Kim, Bum-Joon
• Format: Journal Article
• Language: English
• Published: England 01.07.2015
• Subjects: Animals; Cattle - microbiology; Cattle Diseases - microbiology; Diaminopimelic Acid - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Genes, Bacterial; Molecular Sequence Data; Mycobacterium Infections - microbiology; Mycobacterium Infections - veterinary; Mycolic Acids - chemistry; Nontuberculous Mycobacteria - classification; Nontuberculous Mycobacteria - genetics; Nontuberculous Mycobacteria - isolation & purification; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phylogeny; Pigmentation; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijs.0.000255

From the whole blood of Korean native cattle, Hanwoo ( Bos taurus coreanae ), a previously undescribed, rapidly growing, scotochromogenic isolate of the genus Mycobacterium is reported. Its 16S rRNA gene sequence, and the sequences of three other genes ( hsp65, recA and rpoB ) were unique and phylogenetic analysis based on 16S rRNA gene sequence (1420 bp) placed the organism into the rapidly growing Mycobacterium group close to Mycobacterium smegmatis (98.5 % sequence similarity). However, phylogenetic analyses based on three different gene sequences ( hsp65, recA and rpoB ) revealed its location to be distinct from the branch of rapidly growing species. Culture and biochemical characteristics were generally similar to those of Mycobacterium fortuitum. Unique matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) MS profiles of lipids, unique fatty acid profile, unique mycolic acids profiles and a low DNA–DNA relatedness to M. fortuitum (23.6 %) and M. smegmatis (39.7 %) strongly supported the taxonomic status of this strain as a representative of a novel species of rapidly growing mycobacteria named Mycobacterium anyangense . The type strain is strain QIA-38 T ( = JCM 30275 T  = KCTC 29443 T ).