Production of 12-hydroxyeicosatetraenoic acid and vitamin E status in platelets from type I human diabetic subjects

• Published in: Diabetes (New York, N.Y.) Vol. 34; no. 6; pp. 526 - 531
• Main Authors: Karpen, C.W, Cataland, S, O'Dorisio, T.M, Panganamala, R.V
• Format: Journal Article
• Language: English
• Published: United States 01.06.1985
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Adolescent; Adult; Arachidonic Acid; Arachidonic Acids; Arachidonic Acids - pharmacology; biosynthesis; blood; blood platelets; Blood Platelets - drug effects; Blood Platelets - metabolism; Chromatography, High Pressure Liquid; Collagen; Collagen - pharmacology; diabetes; Diabetes Mellitus, Type 1; Diabetes Mellitus, Type 1 - blood; drug effects; Female; Humans; Hydroxyeicosatetraenoic Acids; Hydroxyeicosatetraenoic Acids - biosynthesis; Male; metabolism; Middle Aged; pharmacology; polyunsaturated fatty acids; Radioimmunoassay; Thrombin; Thrombin - pharmacology; vitamin E; Vitamin E - blood
• ISSN: 0012-1797; 1939-327X
• DOI: 10.2337/diab.34.6.526

Extract: Vitamin E content and biosynthesis of 12-hydroxyeicosatetraenoic acid (12-HETE) have been measured in platelets from type I diabetic subjects and age- and sex-matched, nondiabetic control subjects. Platelets from diabetic subjects synthesized significantly greater quantities of 12-HETE than did platelets from control subjects when 12-HETE synthesis was induced by thrombin or collagen, either in the presence or absence of indomethacin. Platelet conversion of exogenously added arachidonic acid (AA) to 12-HETE was not significantly different between the diabetic and control groups in the absence of indomethacin, although a small but significant increase in the conversion of AA to 12-HETE was present in the diabetic group platelets when indomethacin was added to the reaction. Vitamin E content was significantly reduced in platelets from the diabetic subjects, when compared with platelets from the control subjects, although plasma vitamin E levels were not significantly different between the two groups. Thrombin- and collagen-induced platelet 12-HETE synthesis demonstrated a significant negative linear correlation with platelet vitamin E content when measurements from both diabetic and control groups were combined. The above data suggest a relationship between low vitamin E content and increased 12-HETE synthesis in platelets from type I diabetic subjects.(author)