Prevention and treatment of Schistosoma mansoni-induced liver fibrosis in mice

• Published in: Inflammopharmacology Vol. 19; no. 6; pp. 307 - 316
• Main Authors: El-Agamy, Dina S., Shebl, Abdelhadi M., Said, Shehta A.
• Format: Journal Article
• Language: English
• Published: Basel SP Birkhäuser Verlag Basel 01.12.2011
• Subjects: Alanine Transaminase - blood; Allergology; Animals; Aspartate Aminotransferases - blood; Benzamides; Bilirubin - blood; Biomedical and Life Sciences; Biomedicine; Curcumin - pharmacology; Dermatology; Gastroenterology; Hydroxyproline - metabolism; Imatinib Mesylate; Immunology; Liver - drug effects; Liver - pathology; Liver Cirrhosis - drug therapy; Liver Cirrhosis - parasitology; Liver Cirrhosis - pathology; Liver Cirrhosis - prevention & control; Male; Mice; Pharmacology/Toxicology; Piperazines - pharmacology; Pyrimidines - pharmacology; Research Article; Rheumatology; Schistosoma; Schistosoma mansoni; Schistosoma mansoni - isolation & purification; Schistosomiasis mansoni - blood; Schistosomiasis mansoni - pathology; Serum Albumin - metabolism; Stilbenes - pharmacology; Imatinib; Bosentan; Losartan; Resveratrol; Rosiglitazone; Curcumin; Liver fibrosis
• ISSN: 0925-4692; 1568-5608
• DOI: 10.1007/s10787-011-0092-6

The present study was designed to examine the potential preventive and curative effects of curcumin, resveratrol, imatinib, rosiglitazone, losartan and bosentan (BOS) on Schistosoma mansoni -induced liver fibrosis in mice. Induction of liver fibrosis was produced in male Swiss mice by subcutaneous injection of S. mansoni cercariae per mouse. Mice were left for 28 days before starting the experiment then mice were divided into two main groups. The first group was further subdivided into experimental groups and started drug treatment at day 28 after infection and continued for 2 weeks in order to evaluate the potential preventive effects of the mentioned drugs on S. mansoni -induced liver fibrosis. The second group of mice were left for 2 weeks and then treated with praziquantel for two consecutive days to eradicate the worms and so stop egg disposition and further fibrosis development. Mice were then subdivided into the experimental groups and drug treatment was started for 2 weeks to evaluate their efficacy to decrease the developed fibrosis. At the end of the experiment period, mice were killed and serum was collected for the estimation of alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilirubin and albumin. Liver tissue was taken for the estimation of hepatic hydroxyproline content and histopathological examination to confirm the biochemical results. Results of the study indicate that curcumin and imatinib have potent antifibrotic activity both in suppressing and reversing S. mansoni- induced liver fibrosis, while resveratrol has beneficial effects only in suppressing the development of S. mansoni -induced liver fibrosis.