SarcTrack

• Published in: Circulation research Vol. 124; no. 8; p. 1172
• Main Authors: Toepfer, Christopher N, Sharma, Arun, Cicconet, Marcelo, Garfinkel, Amanda C, Mücke, Michael, Neyazi, Meraj, Willcox, Jon A L, Agarwal, Radhika, Schmid, Manuel, Rao, Jyoti, Ewoldt, Jourdan, Pourquié, Olivier, Chopra, Anant, Chen, Christopher S, Seidman, Jonathan G, Seidman, Christine E
• Format: Journal Article
• Language: English
• Published: United States 12.04.2019
• Subjects: Algorithms; Benzylamines - antagonists & inhibitors; Benzylamines - pharmacology; Cardiovascular Agents - pharmacology; Carrier Proteins - genetics; Clustered Regularly Interspaced Short Palindromic Repeats; Computer-Aided Design; Fluorescence; Humans; Induced Pluripotent Stem Cells - drug effects; Induced Pluripotent Stem Cells - physiology; Microscopy, Atomic Force - methods; Myocardial Contraction; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - physiology; Myosins - drug effects; Myosins - metabolism; Propranolol - pharmacology; Sarcomeres - physiology; Software; Uracil - analogs & derivatives; Uracil - antagonists & inhibitors; Uracil - pharmacology; Urea - analogs & derivatives; Urea - pharmacology; Verapamil - pharmacology; MYK-461; cardiomyocyte contractility; myosin binding protein-C; cell imaging; hypertrophic cardiomyopathy; induced pluripotent stem cells; sarcomeres

Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in combination with CRISPR/Cas9 genome editing provide unparalleled opportunities to study cardiac biology and disease. However, sarcomeres, the fundamental units of myocyte contraction, are immature and nonlinear in hiPSC-CMs, w...