Tracing Baculovirus AcMNPV Infection Using a Real-Time Method Based on ANCHORTM DNA Labeling Technology

• Published in: Viruses Vol. 12; no. 1; p. 50
• Main Authors: Hinsberger, Aurélie, Graillot, Benoît, Blachère Lopez, Christine, Juliant, Sylvie, Cerutti, Martine, King, Linda A., Possee, Robert D., Gallardo, Franck, Lopez Ferber, Miguel
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 02.01.2020; MDPI
• Subjects: baculovirus infection; Cell culture; Cloning; Deoxyribonucleic acid; DNA; DNA-binding protein; Environmental impact; fluorescence labelling; Gene expression; Gene loci; Genomes; Infections; Labeling; Life cycles; Nucleotide sequence; Occlusion bodies; Plasmids; Proteins; real-time imaging; Viruses; France; United States > US

Many steps in the baculovirus life cycle, from initial ingestion to the subsequent infection of all larval cells, remain largely unknown; primarily because it has hitherto not been possible to follow individual genomes and their lineages. Use of ANCHORTM technology allows a high intensity fluorescen...