Mapping of the gene coding for a paraneoplastic encephalomyelitis antigen (HuD) to human chromosome site 1p34

A cDNA clone coding for a human brain protein (HuD), which is highly homologous to the Drosophila neuronal determination protein Elav and elicits antibody formation in a high proportion of patients with paraneoplastic encephalomyelitis, was used to isolate a lambda phage recombinant clone, including...

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Bibliographic Details
Published inCytogenetics and cell genetics Vol. 65; no. 3; p. 177
Main Authors Muresu, R, Baldini, A, Gress, T, Posner, J B, Furneaux, H M, Siniscalco, M
Format Journal Article
LanguageEnglish
Published Switzerland 01.01.1994
Subjects
Chromosome Mapping
Chromosomes, Human, Pair 1
DNA Probes
ELAV Proteins
ELAV-Like Protein 4
Humans
In Situ Hybridization, Fluorescence
Nerve Tissue Proteins - genetics
RNA-Binding Proteins - genetics
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Summary:A cDNA clone coding for a human brain protein (HuD), which is highly homologous to the Drosophila neuronal determination protein Elav and elicits antibody formation in a high proportion of patients with paraneoplastic encephalomyelitis, was used to isolate a lambda phage recombinant clone, including a large fragment of the relevant human genomic region. The fragment proved to be an efficient probe for the precise subregional mapping of the gene by molecular in situ hybridization onto extended human prometaphase chromosomes. Analysis of banded metaphases with clear hybridization signals pointed unequivocally to the localization of the HuD gene to human chromosome band 1p34.
ISSN:0301-0171
DOI:10.1159/000133626