comparative study on the transformation of Aspergillus nidulans by microprojectile bombardment of conidia and a more conventional procedure using protoplasts treated with polyethyleneglycol

• Published in: Applied microbiology and biotechnology Vol. 45; no. 3; pp. 333 - 337
• Main Authors: Herzog, R.W, Daniell, H, Singh, N.K, Lemke, P.A
• Format: Journal Article
• Language: English
• Published: 1996
• Subjects: Biological Sciences; plant breeding; plant genetics

An Aspergillus nidulans strain, auxotrophic for pyrimidine, was transformed to prototrophy by means of microprojectile bombardment. The transformation frequency was somewhat lower than conventional polyethyleneglycol-mediated transformation of protoplasts. However, the percentage of stable transform...