Mapping the substrate-binding subsite specificity of a Porphyromonas gingivalis Tpr peptidase

• Published in: Acta biochimica Polonica Vol. 70; no. 4; p. 963
• Main Authors: Staniec, Dominika, Rut, Wioletta, Drag, Marcin, Burmistrz, Michal, Kitching, Michael, Potempa, Jan
• Format: Journal Article
• Language: English
• Published: Poland 08.12.2023
• Subjects: Calpain - genetics; Calpain - metabolism; Endopeptidases - metabolism; Peptide Hydrolases - metabolism; Porphyromonas gingivalis - genetics; Porphyromonas gingivalis - metabolism; Substrate Specificity

Calcium-dependent peptidases of the calpain family are widespread in eukaryotes but uncommon in prokaryotes. A few bacterial calpain homologs have been discovered but none of them have been characterized in detail. Here we present an in-depth substrate specificity analysis of the bacterial calpain-l...