Chromosomal mosaicism in neural stem cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 438; p. 197
• Main Authors: Peterson, Suzanne E, Westra, Jurjen W, Paczkowski, Christine M, Chun, Jerold
• Format: Journal Article
• Language: English
• Published: United States 2008
• Subjects: Animals; Cell Culture Techniques - methods; Cell Separation; Cells, Cultured; Chromosomes - genetics; Female; Humans; Metaphase; Mice; Mosaicism; Neurons - cytology; Neurons - metabolism; Pregnancy; Spectral Karyotyping; Stem Cells - cytology; Stem Cells - metabolism

Neural stem and progenitor cells (referred to here as NSCs), located in the proliferative zones of embryonic brains, can be seen undergoing mitosis at the ventricular surface. Mitotic NSCs can be arrested in metaphase and chromosome "spreads" produced to reveal their chromosomal complement...