Antidepressants Accumulate in Lipid Rafts Independent of Monoamine Transporters to Modulate Redistribution of the G Protein, Gαs

• Published in: The Journal of biological chemistry Vol. 291; no. 38; pp. 19725 - 19733
• Main Authors: Erb, Samuel J., Schappi, Jeffrey M., Rasenick, Mark M.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.09.2016; American Society for Biochemistry and Molecular Biology
• Subjects: Animals; Antidepressive Agents - pharmacology; Cell Line, Tumor; Chromogranins - genetics; Chromogranins - metabolism; cyclic AMP (cAMP); Cyclic AMP - genetics; Cyclic AMP - metabolism; depression; drug action; G protein; G protein-coupled receptor (GPCR); gas chromatography-mass spectrometry (GC-MS); glia; glial cell; GTP-Binding Protein alpha Subunits, Gs - genetics; GTP-Binding Protein alpha Subunits, Gs - metabolism; heterotrimeric G protein; intracellular trafficking; lipid; lipid raft; mass spectrometry (MS); Membrane Microdomains - genetics; Membrane Microdomains - metabolism; membrane trafficking; monoamine transporter; Papers of the Week; plasma membrane; protein translocation; Protein Transport - drug effects; Protein Transport - physiology; protein-drug interaction; protein-lipid interaction; Rats; Second Messenger Systems - drug effects; Second Messenger Systems - physiology; selective serotonin reuptake inhibitor (SSRI); serotonin; Serotonin Plasma Membrane Transport Proteins - genetics; Serotonin Plasma Membrane Transport Proteins - metabolism; serotonin transporter; plasma membrane; protein-drug interaction; selective serotonin reuptake inhibitor (SSRI); monoamine transporter; intracellular trafficking; cyclic AMP (cAMP); glia; lipid; protein translocation; serotonin; glial cell; lipid raft; gas chromatography-mass spectrometry (GC-MS); protein-lipid interaction; serotonin transporter; drug action; G protein-coupled receptor (GPCR); depression; membrane trafficking; G protein; heterotrimeric G protein; mass spectrometry (MS)

Depression is a significant public health problem for which currently available medications, if effective, require weeks to months of treatment before patients respond. Previous studies have shown that the G protein responsible for increasing cAMP (Gαs) is increasingly localized to lipid rafts in de...