Multiple mechanisms of termination modulate the dynamics of RNAPI transcription

• Published in: Cell reports (Cambridge) Vol. 44; no. 3; p. 115325
• Main Authors: Petfalski, Elisabeth, Winz, Marie-Luise, Grelewska-Nowotko, Katarzyna, Turowski, Tomasz W., Tollervey, David
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.03.2025
• Subjects: biophysical modeling; DNA torsion; Exoribonucleases - metabolism; exosome; ribosome synthesis; RNA polymerase; RNA Polymerase I - genetics; RNA Polymerase I - metabolism; RNA-protein interactions; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; topoisomerase; transcription; Transcription Termination, Genetic; Transcription, Genetic; UV crosslinking; yeast; ribosome synthesis; transcription; RNA-protein interactions; UV crosslinking; biophysical modeling; CP: Molecular biology; topoisomerase; RNA polymerase; exosome; DNA torsion; yeast

Transcription elongation is stochastic, driven by a Brownian ratchet, making it subject to changes in velocity. On the rDNA, multiple polymerases are linked by “torsional entrainment” generated by DNA rotation. We report that release of entrainment by co-transcriptional 3′ end cleavage, is permissiv...