E. coli cells expressing the Baeyer-Villiger monooxygenase 'MO14' (ro03437) from Rhodococcus jostii RHA1 catalyse the gram-scale resolution of a bicyclic ketone in a fermentor

• Published in: Organic & biomolecular chemistry Vol. 13; no. 6; pp. 1897 - 1903
• Main Authors: Summers, Benjamin D, Omar, Muhiadin, Ronson, Thomas O, Cartwright, Jared, Lloyd, Michael, Grogan, Gideon
• Format: Journal Article
• Language: English
• Published: England 14.02.2015
• Subjects: Biocatalysis; Bioreactors; Escherichia coli - cytology; Escherichia coli - genetics; Escherichia coli - metabolism; Gene Expression Regulation, Enzymologic - genetics; Ketones - chemistry; Ketones - metabolism; Molecular Conformation; Oxygenases - chemistry; Oxygenases - genetics; Oxygenases - metabolism; Rhodococcus - enzymology; Rhodococcus - metabolism; Substrate Specificity

The Baeyer-Villiger monooxygenase (BVMO) 'MO14' from Rhodococcus jostii RHA1, is an enantioselective BVMO that catalyses the resolution of the model ketone substrate bicyclo[3.2.0]hept-2-en-6-one to the (1S,5R)-2-oxa lactone and the residual (1S,5R)-substrate enantiomer. This regio-plus en...