Multispectral laser-induced fluorescence imaging system for large biological samples

A laser-induced fluorescence imaging system developed to capture multispectral fluorescence emission images simultaneously from a relatively large target object is described. With an expanded, 355-nm Nd:YAG laser as the excitation source, the system captures fluorescence emission images in the blue,...

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Bibliographic Details
Published inApplied optics (2004) Vol. 42; no. 19; p. 3927
Main Authors Kim, Moon S, Lefcourt, Alan M, Chen, Yud-Ren
Format Journal Article
LanguageEnglish
Published United States 01.07.2003
Subjects
Animals
Cattle
Equipment Design
Equipment Failure Analysis
Feces - chemistry
Food Contamination - analysis
Fruit - chemistry
Lasers
Malus - chemistry
Meat - analysis
Sensitivity and Specificity
Spectrometry, Fluorescence - instrumentation
Spectrometry, Fluorescence - methods
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Summary:A laser-induced fluorescence imaging system developed to capture multispectral fluorescence emission images simultaneously from a relatively large target object is described. With an expanded, 355-nm Nd:YAG laser as the excitation source, the system captures fluorescence emission images in the blue, green, red, and far-red regions of the spectrum centered at 450, 550, 678, and 730 nm, respectively, from a 30-cm-diameter target area in ambient light. Images of apples and of pork meat artificially contaminated with diluted animal feces have demonstrated the versatility of fluorescence imaging techniques for potential applications in food safety inspection. Regions of contamination, including sites that were not readily visible to the human eye, could easily be identified from the images.
ISSN:1559-128X
2155-3165
DOI:10.1364/AO.42.003927