Analysis of a cell-free DNA–based cancer screening cohort links fragmentomic profiles, nuclease levels, and plasma DNA concentrations

• Published in: Genome research Vol. 35; no. 1; pp. 31 - 42
• Main Authors: Malki, Yasine, Kang, Guannan, Lam, W.K. Jacky, Zhou, Qing, Cheng, Suk Hang, Cheung, Peter P.H., Bai, Jinyue, Chan, Ming Lok, Lee, Chui Ting, Peng, Wenlei, Zhang, Yiqiong, Gai, Wanxia, Wong, Winsome W.S., Ma, Mary-Jane L., Li, Wenshuo, Xu, Xinzhou, Gao, Zhuoran, Tse, Irene O.L., Shang, Huimin, Choy, L.Y. Lois, Jiang, Peiyong, Chan, K.C. Allen, Lo, Y.M. Dennis
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.01.2025
• Subjects: Adult; Aged; Biopsy; Cancer screening; Cell-Free Nucleic Acids - blood; Cell-Free Nucleic Acids - genetics; Cohort Studies; Deoxyribonucleic acid; DNA; Early Detection of Cancer - methods; Endodeoxyribonucleases - blood; Female; Fetuses; Humans; Male; Middle Aged; Neoplasms - blood; Neoplasms - diagnosis; Neoplasms - genetics; Nuclease; Plasma; Tumors

The concentration of circulating cell-free DNA (cfDNA) in plasma is an important determinant of the robustness of liquid biopsies. However, biological mechanisms that lead to inter-individual differences in cfDNA concentrations remain unexplored. The concentration of plasma cfDNA is governed by an i...