First report on CRISPR/Cas9 mediated editing of the eye colour gene, Tryptophan 2, 3-dioxygenase in egg plant shoot and fruit borer Leucinodes orbonalis Guenée (Lepidoptera: Crambidae)

• Published in: Journal of Asia-Pacific entomology Vol. 26; no. 1; pp. 102031 - 10
• Main Authors: Ashok, Karuppannasamy, Bhargava, Chikmagalur Nagaraja, Prasad Babu, Karakatti, Rohan, Wishard, Manamohan, Malligopal, Rai, Anil, Sanjay, Kumar Pradhan, Parvathy, Madhusoodanan Sujatha, Kennedy, John Samuel, Asokan, Ramasamy
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.03.2023; 한국응용곤충학회
• Subjects: 3-dioxygenase; color; CRISPR-associated endonuclease 9; CRISPR-Cas systems; CRISPR/Cas9; eggplants; entomology; eyes; fruits; genes; Genome editing; Insect eye pigment; Leucinodes orbonalis; mutants; phenotype; ribonucleoproteins; sex determination; spermatogenesis; sterile insect technique; transgenesis; tryptophan; Tryptophan 2; 농학; Genome editing; Leucinodes orbonalis; CRISPR/Cas9; Tryptophan 2; 3-dioxygenase; Insect eye pigment

[Display omitted] •Developed first-time CRISPR/Cas9 mediated eye colour edit in Leucinodes orbonalis.•Tryptophan 2,3 dioxygenase is the first enzyme of ommochrome pathway in eye colour.•Transcribed gRNA from fifth exon of the above gene, formed RNP complex with Cas9.•RNP was microinjected into G0 st...