In Vitro Propagation of Artemisia japonica

An effective protocol for in vitro propagation was developed for Artemisia japonica using nodal explants. The efficiency of the explants to induce the multiple shoots on Murashige and Skoog (MS) medium supplemented with 2.5, 5.0, 7.5, and 10 µM of indole-3-acetic acid (IAA), indole-3-butyric acid (I...

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Published inJournal of herbs, spices & medicinal plants Vol. 23; no. 1; pp. 36 - 43
Main Authors Shinde, Smita, Katewal, Pooja R., Shanbhag, Divya D., Joseph, Kadanthottu Sebastian, Murthy, H. N.
Format Journal Article
LanguageEnglish
Published Philadelphia Taylor & Francis 02.01.2017
Taylor & Francis Ltd
Subjects
Acclimatization
Artemisia
axillary shoots
Botany
micropropagation
Plant growth
Propagation
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Summary:An effective protocol for in vitro propagation was developed for Artemisia japonica using nodal explants. The efficiency of the explants to induce the multiple shoots on Murashige and Skoog (MS) medium supplemented with 2.5, 5.0, 7.5, and 10 µM of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), 1-napthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), 6-benzylaminopurine (BAP), thidiazuron (TDZ), kinetin (Kn), and 2-isopentenyl adenine (2iP) was tested. A maximum of 20 axillary shoots per explant were induced on MS medium supplemented with 10 µM BAP. Shoots were rooted on MS medium supplemented with IBA (10 µM). The resulting plantlets were hardened in a mixture of cocopeat and perlite (1:1). Cytological analysis revealed that regenerated plants had 2n = 18 chromosomes, which was same as that of the mother plant.
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ISSN:1049-6475
1540-3580
DOI:10.1080/10496475.2016.1256010