Ganglioside sialidase activity in bovine neuronal perikarya
Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya an...
Saved in:
| Published in | Neurochemical research Vol. 1; no. 2; p. 171 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
United States
01.04.1976
|
| Online Access | Get more information |
Cover
Loading…
| Abstract | Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the homogenate. Synaptosomes had 26-33 nmol total sialic acid/mg protein, while the neurons had only 15-17 nmol. Determination of ganglioside sialidase activity showed that neuronal perikarya had very low levels (negligible), in comparison with synaptosomes or whole-brain homogenates. Trypsin treatment during the isolation procedure enhanced sialidase activity two-to threefold in the particulate fraction of the whole-brain homogenate. Determination of the distribution of sialidase activity in the fractions obtained during the isolation of the neuronal perikarya showed that the sialidase activity was associated with the myelin, broken-off dendritic processes, and glial-cell fractions that banded in the less dense sucrose. |
|---|---|
| AbstractList | Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar ganglioside patterns, with the synaptosomes containing at least four times more total ganglioside per mg protein than the neuronal perikarya and twice that of the homogenate. Synaptosomes had 26-33 nmol total sialic acid/mg protein, while the neurons had only 15-17 nmol. Determination of ganglioside sialidase activity showed that neuronal perikarya had very low levels (negligible), in comparison with synaptosomes or whole-brain homogenates. Trypsin treatment during the isolation procedure enhanced sialidase activity two-to threefold in the particulate fraction of the whole-brain homogenate. Determination of the distribution of sialidase activity in the fractions obtained during the isolation of the neuronal perikarya showed that the sialidase activity was associated with the myelin, broken-off dendritic processes, and glial-cell fractions that banded in the less dense sucrose. |
| Author | Schengrund, C L Nelson, J T |
| Author_xml | – sequence: 1 givenname: C L surname: Schengrund fullname: Schengrund, C L organization: Department of Biological Chemistry The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania – sequence: 2 givenname: J T surname: Nelson fullname: Nelson, J T |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/24271340$$D View this record in MEDLINE/PubMed |
| BookMark | eNo1j81KxDAUhbMYcX504wNIXqB6c5O2Ka50cEZhwI2uh5vmVqKdtDSdgXl7C-rqcODjfJylmMUushA3Cu4UQHn_tAGoikKBnYkF6MJkWlUwF8uUvgAmBNWlmKPBUmkDC_GwpfjZhi4FzzIFaoOnxJLqMZzCeJYhStedQmQZ-Th0kVrZ8xC-aTjTlbhoqE18_Zcr8bF5fl-_ZLu37ev6cZfVaPMxc74hXRnLDl3jwUxNQ23QAxMZ6xV7X1hwRuVUMhlEp5rSI-Zkcl3UuBK3v7v90R3Y7_shHCb__v8F_gC1VUj- |
| CitedBy_id | crossref_primary_10_1002_jnr_490400315 crossref_primary_10_1016_0197_0186_92_90187_V crossref_primary_10_1111_j_1471_4159_1977_tb10737_x crossref_primary_10_1016_S0163_7827_97_00008_8 crossref_primary_10_1111_j_1471_4159_1989_tb11756_x crossref_primary_10_1111_j_1471_4159_1992_tb09279_x crossref_primary_10_1111_j_1471_4159_1989_tb08539_x crossref_primary_10_1007_BF00966109 |
| ContentType | Journal Article |
| DBID | NPM |
| DOI | 10.1007/BF00966108 |
| DatabaseName | PubMed |
| DatabaseTitle | PubMed |
| DatabaseTitleList | PubMed |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database |
| DeliveryMethod | no_fulltext_linktorsrc |
| Discipline | Anatomy & Physiology |
| ExternalDocumentID | 24271340 |
| Genre | Journal Article |
| GroupedDBID | --- -4W -56 -5G -BR -EM -Y2 -~C -~X .55 .86 .GJ .VR 06C 06D 0R~ 0VY 123 199 1N0 1SB 2.D 203 28- 29N 29~ 2J2 2JN 2JY 2KG 2KM 2LR 2P1 2VQ 2~H 30V 3SX 3V. 4.4 406 408 409 40D 40E 53G 5QI 5VS 67N 67Z 6NX 78A 7X7 88A 88E 8AO 8FE 8FH 8FI 8FJ 8TC 8UJ 95- 95. 95~ 96X AAAVM AABHQ AACDK AAEOY AAHNG AAIAL AAJBT AAJKR AANXM AANZL AAQLM AARHV AARTL AASML AATNV AATVU AAUYE AAWCG AAYIU AAYQN AAYTO AAYZH ABAKF ABBBX ABBXA ABDZT ABECU ABFTV ABHLI ABHQN ABJNI ABJOX ABKCH ABKTR ABMNI ABMQK ABNWP ABPLI ABQBU ABSXP ABTEG ABTHY ABTKH ABTMW ABULA ABUWG ABWNU ABXPI ACAOD ACBXY ACDTI ACGFS ACHSB ACHXU ACIPQ ACIWK ACKNC ACMDZ ACMLO ACOKC ACOMO ACPRK ACZOJ ADBBV ADHHG ADHIR ADIMF ADINQ ADKNI ADKPE ADRFC ADTPH ADURQ ADYFF ADYPR ADZKW AEBTG AEFIE AEFQL AEGAL AEGNC AEJHL AEJRE AEKMD AEMSY AENEX AEOHA AEPYU AESKC AETLH AEVLU AEXYK AFBBN AFEXP AFFNX AFGCZ AFKRA AFLOW AFQWF AFRAH AFWTZ AFZKB AGAYW AGDGC AGGDS AGJBK AGMZJ AGQEE AGQMX AGRTI AGWIL AGWZB AGYKE AHAVH AHBYD AHKAY AHMBA AHSBF AHYZX AIAKS AIGIU AIIXL AILAN AITGF AJBLW AJRNO AJZVZ AKMHD ALIPV ALMA_UNASSIGNED_HOLDINGS ALWAN AMKLP AMXSW AMYLF AMYQR AOCGG ARMRJ ASPBG AVWKF AXYYD AZFZN B-. BA0 BBNVY BBWZM BDATZ BENPR BGNMA BHPHI BPHCQ BVXVI CAG CCPQU COF CS3 CSCUP DDRTE DL5 DNIVK DPUIP DU5 EBD EBLON EBS EIOEI EJD EMOBN EN4 EPAXT ESBYG F5P FEDTE FERAY FFXSO FIGPU FINBP FNLPD FRRFC FSGXE FWDCC FYUFA G-Y G-Z GGCAI GGRSB GJIRD GNWQR GQ6 GQ7 GQ8 GXS H13 HCIFZ HF~ HG5 HG6 HMCUK HMJXF HQYDN HRMNR HVGLF HZ~ I09 IHE IJ- IKXTQ ITM IWAJR IXC IZIGR IZQ I~X I~Z J-C J0Z JBSCW JCJTX JZLTJ KDC KOV KOW KPH LAK LK8 LLZTM M0L M1P M4Y M7P MA- N2Q N9A NB0 NDZJH NPM NPVJJ NQJWS NU0 O9- O93 O9G O9I O9J OAM OVD P19 P2P PF0 PQQKQ PROAC PSQYO PT4 PT5 Q2X QOK QOR QOS R4E R89 R9I RHV RNI RNS ROL RPX RRX RSV RZC RZE RZK S16 S1Z S26 S27 S28 S3A S3B SAP SBL SBY SCLPG SDH SDM SHX SISQX SJYHP SNE SNPRN SNX SOHCF SOJ SPISZ SRMVM SSLCW SSXJD STPWE SV3 SZN T13 T16 TEORI TSG TSK TSV TUC U2A U9L UG4 UKHRP UOJIU UTJUX UZXMN VC2 VFIZW W23 W48 WJK WK6 WK8 X7M XJT YLTOR Z45 Z7U Z82 Z83 Z87 Z8O Z8V Z91 ZGI ZMTXR ZOVNA ~EX |
| ID | FETCH-LOGICAL-c285t-bdfa3948eb2bfd04fa330c42d0eaa48d1edd680b415a7ea422b1f7d225a4536c2 |
| ISSN | 0364-3190 |
| IngestDate | Tue Oct 15 23:49:23 EDT 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 2 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c285t-bdfa3948eb2bfd04fa330c42d0eaa48d1edd680b415a7ea422b1f7d225a4536c2 |
| PMID | 24271340 |
| ParticipantIDs | pubmed_primary_24271340 |
| PublicationCentury | 1900 |
| PublicationDate | 1976-04-00 |
| PublicationDateYYYYMMDD | 1976-04-01 |
| PublicationDate_xml | – month: 04 year: 1976 text: 1976-04-00 |
| PublicationDecade | 1970 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States |
| PublicationTitle | Neurochemical research |
| PublicationTitleAlternate | Neurochem Res |
| PublicationYear | 1976 |
| References | 4257325 - Biochim Biophys Acta. 1971 Dec 3;249(2):380-94 4994328 - Biochemistry. 1970 Sep 15;9(19):3774-82 13918693 - Dan Med Bull. 1962 Mar;9:16-7 13782493 - Biochem Z. 1961;334:175-84 4355577 - J Biol Chem. 1973 Nov 10;248(21):7353-8 5840391 - Biochem J. 1964 Jul;92(1):91-100 6025100 - J Neurochem. 1967 Jun;14(6):671-4 13436486 - Biochim Biophys Acta. 1957 Jun;24(3):604-11 4359476 - J Membr Biol. 1973 Dec 6;14(1):73-84 14907713 - J Biol Chem. 1951 Nov;193(1):265-75 5086244 - J Neurochem. 1972 Nov;19(11):2567-75 4373537 - J Neurochem. 1974 Nov;23(5):1025-36 5118336 - J Neurochem. 1971 Oct;18(10):1821-9 5542708 - J Lipid Res. 1971 Jan;12(1):84-90 14861227 - J Biol Chem. 1951 Aug;191(2):819-31 4305523 - Biochim Biophys Acta. 1969 Feb 18;177(1):1-10 4642578 - Brain Res. 1972 Dec 12;47(2):515-8 4337100 - J Biol Chem. 1972 May 10;247(9):2742-6 5428405 - Nature. 1970 Jul 11;227(5254):170-1 13672998 - J Biol Chem. 1959 Aug;234(8):1971-5 5679970 - J Biol Chem. 1968 Aug 25;243(16):4380-90 4351222 - J Biol Chem. 1973 Jun 25;248(12):4424-8 5484473 - J Biol Chem. 1970 Nov 25;245(22):6196-200 14231772 - Life Sci (1962). 1964 Nov;3:1227-33 5542012 - J Biol Chem. 1971 Jan 25;246(2):430-5 1125012 - Biochem Biophys Res Commun. 1975 Mar 3;63(1):217-23 4904192 - Science. 1970 Feb 20;167(3921):1144-5 14253569 - J Neuropathol Exp Neurol. 1965 Jan;24:130-5 5027747 - J Biol Chem. 1972 May 25;247(10):3159-69 |
| References_xml | |
| SSID | ssj0010021 |
| Score | 1.25592 |
| Snippet | Neuronal perikarya were isolated, using bulk preparative procedures, from bovine brains. Synaptosomes, neuronal perikarya, and brain homogenates had similar... |
| SourceID | pubmed |
| SourceType | Index Database |
| StartPage | 171 |
| Title | Ganglioside sialidase activity in bovine neuronal perikarya |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/24271340 |
| Volume | 1 |
| hasFullText | |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bT8IwFG5EX3wxKt4v6YPxhczs0q1dfCJGJSTyIiS8kXbdDDEMgvMBf72n7Tomary8LLCSwfptZ9859PsOQhckgnsm467jkQwSlAguYy49SFwDTgNKGZG6NPDQizoD0h2Gw2VHQa0uKcRV8valruQ_qMI-wFWpZP-AbHVQ2AGvAV_YAsKw_RXG91yJcKeq42brBb5uLOGZpO0xdEeIcd4SqmCQtrRrpeKdytf4mc8XvE5KtUFHYp0DSvufqkz8CCP50_zVVKCtTEHXj63TY7dcai2NnI5GtUUnVjVFIA6blp1VRKwB79eim2e6pXyKuq5dSw75ENAxVv8QzNhsoucfyIASrro_j644YNuhBmpQptpz9FRFxnapcLW2rjqPjxa05Q9Sls_lQVbSB00j-ttoq-T_uG3A3EFrab6Lmu2cF9PJAl9ivSJX_9XRRNc1fHGFL7b44nGODb7Y4osrfPfQ4O62f9NxymYXTuKzsHCEzHgQE5YKX2TSJfAucBPiSzflnDDppVJGzBVAuDhNOfF94WVUQjjmJAyixN9H6_k0Tw8RpippFHGYJWFEPMpiIHFw5kCHgNsFMTtCB2YKRjPjaDKyk3P87cgJ2lxePqdoI4NbKD0DPlaIcw3IO9MAMiE |
| link.rule.ids | 780 |
| linkProvider | National Library of Medicine |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Ganglioside+sialidase+activity+in+bovine+neuronal+perikarya&rft.jtitle=Neurochemical+research&rft.au=Schengrund%2C+C+L&rft.au=Nelson%2C+J+T&rft.date=1976-04-01&rft.issn=0364-3190&rft.volume=1&rft.issue=2&rft.spage=171&rft_id=info:doi/10.1007%2FBF00966108&rft_id=info%3Apmid%2F24271340&rft_id=info%3Apmid%2F24271340&rft.externalDocID=24271340 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0364-3190&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0364-3190&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0364-3190&client=summon |