Anti-Inflammatory Activity of Vacuum Distillate from Panax ginseng Root on LPS-Induced RAW264.7 Cells

• Published in: Journal of microbiology and biotechnology Vol. 34; no. 2; pp. 262 - 269
• Main Authors: Lee, Chanwoo, Lee, Seul, Jang, Young Pyo, Park, Junseong
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국미생물·생명공학회 28.02.2024
• Subjects: 생물학; MAPK pathway; Anti-inflammation; ginseng distillate; NF-κB pathway
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.2312.12001

has been widely applied as an important herb in traditional medicine to treat numerous human disorders. However, the inflammatory regulation effect of distillate (GSD) has not yet been fully assessed. To determine whether GSD can ameliorate inflammatory processes, a GSD was prepared using the vacuum distillation process for the first time, and the regulation effect on lipopolysaccharide-induced macrophages was assessed. The results showed that GSD effectively inhibited nitric oxide (NO) formation and activation of inducible nitric oxide synthase (iNOS) mRNA in murine macrophage cell, but not cyclooxy-genase-2 production. The mRNA expression pattern of tumor necrosis factor alpha and IL-6 were also reduced by GSD. Furthermore, we confirmed that GSD exerted its anti-inflammatory effects by downregulating c-Jun NH2-terminal kinase (JNK) phosphorylation, the extracellular signal-regulated kinase phosphorylation, and signaling pathway of nuclear factor kappa B (NF-κB). Our findings revealed that the inflammatory regulation activity of GSD could be induced by iNOS and NO formation inhibition mediated by regulation of nuclear factor kappa B and p38/JNK MAPK pathways.