Cross-linking of the DNA repair protein O6-alkylguanine DNA alkyltransferase to DNA in the presence of cisplatin

• Published in: DNA repair Vol. 89; p. 102840
• Main Authors: Ming, Xun, Michaelson-Richie, Erin D., Groehler, Arnold S., Villalta, Peter W., Campbell, Colin, Tretyakova, Natalia Y.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2020
• Subjects: AGT; Cisplatin; DNA-protein cross-links; Mass spectrometry; DEB; dG-Pt-Lys; dGpG; DPC; HPLC-ESI+-MS/MS; DNA-protein cross-links; GAPDH; Mass spectrometry; Cisplatin; AGT

•Cisplatin cross-links DNA repair protein O6-Alkylguanine DNA Alkyltransferase to DNA.•Cross-linking sites include Glu110, Lys125, Cys145, His146, Arg147, and Cys150.•Upon heating, dG-Pt-AGT complexes undergo platination migration from protein to DNA to form guanine-guanine cross-links. 1,1,2,2-cis-...