Detection of Small Amounts of Yests, Bacteria and Phages Particles as Biological Objects in Agarose Gel Samples Using Uncoated Plate Acoustic Wave Sensor

• Published in: Procedia technology Vol. 27; pp. 268 - 270
• Main Authors: Anisimkin, Vladimir I., Kuznetsova, Iren E., Kolesov, Vladimir V., Sorokin, Vladimir V., Skladnev, Dimitry A., Caliendo, Cinzia, Verona, Enrico
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 2017
• Subjects: acoustic biosensor; actinobacteria Dietzia sp. cells; agarose hydrogel; G7C coliphage particles; piezoactive plate acoustic waves; yeasts Yarrowia lipolytica cells; agarose hydrogel; yeasts Yarrowia lipolytica cells; acoustic biosensor; piezoactive plate acoustic waves; G7C coliphage particles; actinobacteria Dietzia sp. cells
• DOI: 10.1016/j.protcy.2017.04.114
• ISSN: 2212-0173

Capabilities of the acoustic wave sensing without using special sorbent films are studied at normal conditions with the use of three types of biological objects to detect coliphages, bacteria and yeasts in ordinary liquid rich growth Luria-Bertani media as well as for these biological objects immobilized in agarose hydrogel in various concentrations (and in highly diluted Luria-Bertani broth as reference media also). The method does not require special bath and large volume of test samples (∼100 μL), demonstrates high long-term stability, but possesses some inherent limitations to be discussed. Dilution of the analytes in the reference media is detected to change electric conductivity, but not viscosity of the suspension. The values of the conductivity σ are around 0.5 S/m. The minimum detectable bacteria concentration is estimated as 100 - 1000 cell/ml.