Comparing skin swabs, buccal swabs, and toe clips for amphibian genetic sampling, a case study with a small anuran ( Acris blanchardi )

• Published in: Biology methods and protocols Vol. 9; no. 1; p. bpae030
• Main Authors: Rainey, Travis A, Tryc, Emily E, Nicholson, Kirsten E
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 11.01.2024
• Subjects: Methods; Blanchard’s Cricket Frog; non-lethal sampling; DNA extraction; non-invasive sampling; genotyping

Multiple methods for collecting genetic samples from amphibians exist, each with their own implications for study design, animal welfare, and costs. Toe clipping is one common method, but there is ongoing debate regarding its potential detriment. Less invasive methods should be implemented, if effic...