From colorless to pink: Structural insights into vitamin B12-induced color change in monoclonal antibodies

• Published in: Medicine in drug discovery Vol. 28; p. 100224
• Main Authors: He, Kai-feng, Cui, Hao-dong, Deng, Wen-hui, Xing, Na, Liu, Guo-jian, Chaurembo, Abdallah Iddy, Fu, Li-dan, Li, Yuan, Tong, Xin-yue, Lin, Han-bin, Wang, Chun-he
• Format: Journal Article
• Language: English
• Published: Elsevier 01.12.2025
• Subjects: Antibody color; Cysteine; Protein pocket; Vitamin B12

Background: Monoclonal antibodies (mAbs) are typically colorless; however, the pink coloration observed during production raises quality concerns. This study investigated the mechanism linking this color change to Vitamin B12 (VB12), which converts to hydroxycobalamin (OH-Cbl) under light and binds...