Flow cytometric analysis of whole blood lysis, three anticoagulants, and five cell preparations

• Published in: Cytometry (New York, N.Y.) Vol. 13; no. 1; pp. 68 - 74
• Main Authors: Carter, Patricia H., Resto‐Ruiz, Sandra, Washington, Glennelle C., Ethridge, Steve, Palini, Alessio, Vogt, Robert, Waxdal, Myron, Fleisher, Thomas, Noguchi, Philip D., Marti, Gerald. E.
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 1992; Wiley-Liss
• Subjects: ACD; acid citrate dextrose; Analysis of complex biological substances; Analytical, structural and metabolic biochemistry; Antigens, CD - analysis; Antigens, Differentiation, Myelomonocytic - analysis; Biological and medical sciences; Blood and biological fluids; Blood Chemical Analysis; Blood Preservation; CD13 Antigens; cell viability; Citric Acid; Edetic Acid; Ethylenediaminetetracetic acid (EDTA); Flow Cytometry; Fundamental and applied biological sciences. Psychology; Glucose - analogs & derivatives; Hemolysis; Heparin; Histocompatibility Antigens - analysis; Humans; immunophenotyping; Leukocyte Common Antigens; Light; Lipopolysaccharide Receptors; Propidium; Scattering, Radiation; Human; Whole blood; Flow cytometry; Biological fluid; Phenotype; Anticoagulant; Viability; Comparative study

We studied the effects of anticoagulants and cell preparation methods on lymphocyte forward‐angle s catter (FSC), autofluorescence, and immunofluorescent staining for CD45, CD14, and CD13. Blood samples collected in ethylenediaminetetracetic acid (EDTA), heparin, and acid citrate dextrose (ACD) were...