Relationship between Insuline-like Growth Factor-Ⅰ and Progesterone Secretion of Cultured Human Trophoblast Cells in Vitro

• Published in: Journal of reproduction and contraception Vol. 18; no. 4; pp. 237 - 245
• Main Authors: ZHANG, Xiao-jin, GUI, Sui-qi, CAO, Lin, SUN, Zu-yue
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2007; Obstetrics and Gynecology Hospital of Fudan University, Shanghai 200011, China%Department of Pharmacology and Toxicology, SIPPR, Shanghai 200032, China
• Subjects: insuline-like growth factor-I; low density lipoprotein receptor (LDLR); progesterone; trophoblast cells; 低蛋白受体; 滋养细胞; 生长因素; 胰岛素; insuline-like growth factor-I; trophoblast cells; low density lipoprotein receptor (LDLR); progesterone; insuline-like growth factor-
• ISSN: 1001-7844
• DOI: 10.1016/S1001-7844(07)60029-7

Objective To investigate the effect of insuline-like growth factor-Ⅰ （IGF-Ⅰ） on progesterone genesis and regulation. Methods Cytotrophoblast cells were collected by trypsin-collagenase digestion and percoll gradient centrifugation for primary culture. After stimulated with different concentrations（100 μg/ml, 10 μg/ml, 1 μg/ml, 0.1 μg/ml） of IGF-Ⅰ at the same time and with different duration（12 h,24 h,48 h, 72 h） of IGF-Ⅰ with the same concentration, progesterone levels in the media were measured by radioimmunoassay. Simultaneously, semiquantitative reverse transcriptase polymerase chain reaction （RT-PCR） was applied to determine the expression of low density lipoprotein receptor （LDLR） mRNA. Results Progesterone levels correlated positively with IGF-Ⅰ along with the IGF-Ⅰ concentration increasing, progesterone level began to increase at 12 h, and reached the climax at 48 h when cultured with 100 μg/L IGF-Ⅰ. The expression of LDLR mRNA was detectable in every group and accordant with variation of progesterone level. Conclusion Progesterone secretion has time- and dose-dependent effect on IGF-Ⅰ, and IGF-1 can up-regulate the expression of LDLR mRNA. IGF-Ⅰ may play an important role in promoting secretion of progesterone in trophoblast cells.