Automated Tools to Advance High-Resolution Imaging in Liquid

• Published in: Microscopy and microanalysis Vol. 28; no. 2; pp. 361 - 370
• Main Authors: Jonaid, G. M., Casasanta, Michael A., Dearnaley, William J., Berry, Samantha, Kaylor, Liam, Dressel-Dukes, Madeline J., Spilman, Michael S., Gray, Jennifer L., Kelly, Deborah F.
• Format: Journal Article
• Language: English
• Published: New York, USA Cambridge University Press 01.04.2022; Oxford University Press
• Subjects: Automation; Biological activity; Biological computing; COVID-19; Data collection; Electron microscopy; Graphene; High resolution; Image resolution; Macromolecules; Medical research; Microscopy; N protein; Nucleocapsids; Oligomerization; Particle size; Proteins; Room temperature; Semiconductors; Severe acute respiratory syndrome coronavirus 2; Silicon nitride; Software; Software and Instrumentation; Specimen preparation; Symmetry; Vaccine development; Viral diseases; COVID-19; liquid-electron microscopy; nucleocapsid protein; SARS-CoV-2; adeno-associated virus

Liquid-electron microscopy (EM), the room-temperature correlate to cryo-EM, is a rapidly growing field providing high-resolution insights of macromolecules in solution. Here, we describe how liquid-EM experiments can incorporate automated tools to propel the field to new heights. We demonstrate fres...