A novel hydrogen peroxide fluorescent probe for bioimaging detection and enables multiple redox cycles

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 276; p. 121218
• Main Authors: Ma, Tao, Fu, Kai, Li, Zhichun, Yuan, Chuangchun, Ma, Wenbing
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 05.08.2022
• Subjects: Bioimaging; Cyclic response; Fluorescent Dyes - chemistry; Fluorescent probe; Glutathione - analysis; Hydrogen peroxide; Hydrogen Peroxide - analysis; Naphthalimides; Oxidation-Reduction; Bioimaging; Hydrogen peroxide; Fluorescent probe; Cyclic response
• ISSN: 1386-1425; 1873-3557
• DOI: 10.1016/j.saa.2022.121218

[Display omitted] •A water-soluble H2O2 probe MNG was developed.•MNG possesses large Stokes shift (135 nm) and a low detection limit.•After MNG oxidized by H2O2, it can be reduced with GSH.•MNG is capable of fluorescence imaging H2O2 in MCF-7 cells andin Argentine Bloodfin. In this subject, a novel hydrogen peroxide (H2O2) fluorescent probe (MNG) was designed and developed using naphthalimide derivatives and selenomorpholine. In PBS buffer (10 mM, pH = 7.4, 1 %DMSO), the selenomorpholine on the probe is capable of qualitatively and quantitatively detecting (H2O2) at a small amount under a detection limit of 61 nM. The probe follows a mechanism that Se (Ⅱ) in selenomorpholine is transformed to Se (Ⅳ), thus changing the spectra of the probe MNG. It is noteworthy that MNG can continuously make a cyclic response to H2O2 and glutathione (GSH), so it can potentially achieve redox process imaging in vivo. Moreover, this subject verified the redox process of the probe’s continuous redox response in the Gaussian 09 programme through simulation calculation and mass spectrometry. The probe exhibits high biocompatibility. Moreover, it can detect H2O2 in MCF-7 cells and Argentine Bloodfin.