The effects of drugs inhibiting protein secretion in the filamentous fungus Trichoderma reesei. Evidence for down-regulation of genes that encode secreted proteins in the stressed cells

• Published in: The Journal of biological chemistry Vol. 278; no. 45; pp. 45011 - 45020
• Main Authors: Pakula, Tiina M, Laxell, Marjukka, Huuskonen, Anne, Uusitalo, Jaana, Saloheimo, Markku, Penttilä, Merja
• Format: Journal Article
• Language: English
• Published: United States 07.11.2003
• Subjects: A23187; beta-Galactosidase - genetics; Biological Transport - drug effects; bip1 gene; Brefeldin A - pharmacology; Calcimycin - pharmacology; Cellulose 1,4-beta-Cellobiosidase - chemistry; Cellulose 1,4-beta-Cellobiosidase - genetics; Cellulose 1,4-beta-Cellobiosidase - metabolism; Dithiothreitol - pharmacology; Feedback, Physiological; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Expression Regulation, Fungal - drug effects; Genes, Reporter - genetics; Ionophores - pharmacology; pdi1 gene; Promoter Regions, Genetic - genetics; Protein Folding; Protein Synthesis Inhibitors - pharmacology; RNA, Messenger - analysis; Signal Transduction; Trichoderma - drug effects; Trichoderma - genetics; Trichoderma - physiology; Trichoderma reesei

To study the mechanisms of protein secretion as well as the cellular responses to impaired protein folding and transport in filamentous fungi, we have analyzed Trichoderma reesei cultures treated with chemical agents that interfere with these processes, dithiothreitol, brefeldin A, and the Ca(2+)-io...