Immunolocalization of melatonin receptor type 1 in the sheep ovary and involvement of the PI3K/Akt/FOXO3a signaling pathway in the effects of melatonin on survival and in vitro activation of primordial follicles

• Published in: Molecular reproduction and development Vol. 89; no. 10; pp. 485 - 497
• Main Authors: Barberino, Ricássio S., Macedo, Taís J. S., Lins, Thae Lanne B. G., Menezes, Vanúzia G., Silva, Regina L. S., Monte, Alane P. O., Palheta, Raimundo C., Smitz, Johan E. J., Matos, Maria Helena T.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2022
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Caspase; Caspase 3 - metabolism; Female; Follicles; Forkhead protein; FOXO3 protein; hormone receptor; Immunohistochemistry; in vitro culture; Kinases; Localization; Melatonin; Melatonin - metabolism; Melatonin - pharmacology; ovarian cortex; Ovaries; Ovary - metabolism; Phosphatidylinositol 3-Kinases - metabolism; Phosphatidylinositols - metabolism; Phosphatidylinositols - pharmacology; Phosphorylation; preantral follicle; Proteins; Proto-Oncogene Proteins c-akt - metabolism; Receptors, Melatonin - metabolism; Sheep; Signal Transduction; Survival; Tissue culture; preantral follicle; immunohistochemistry; hormone receptor; ovarian cortex; in vitro culture

This study characterized the expression of melatonin receptor type 1 (MT1) protein in sheep ovaries, evaluated melatonin effects on primordial follicle survival and development after in vitro culture of ovarian tissue and verified the possible involvement of the phosphatidylinositol‐3‐kinase/protein...