In vitro inflammatory modulation of bioceramic endodontic sealer in macrophages stimulated by bacterial lipopolysaccharide
Aim To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS). Methodology After initial setting, the sealers were conditioned with s...
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Published in | International endodontic journal Vol. 56; no. 2; pp. 213 - 226 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Wiley Subscription Services, Inc
01.02.2023
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Subjects | |
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Abstract | Aim
To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS).
Methodology
After initial setting, the sealers were conditioned with serum‐free culture medium for 24 h (1 ml/cm2). Macrophages from the RAW 264.7 strain were exposed to sealer extracts in a 1:16 ratio in a culture medium with or without LPS. Cell morphology, viability, mitochondrial activity, oxidative stress and gene expression of interleukin 1β (IL‐1β) and tumour necrosis factor‐alpha (TNF‐α) were evaluated. Data on mitochondrial activity, oxidative stress and TNF‐α were analysed using a two‐way analysis of variance (anova) test, followed by the Student–Newman–Keuls post‐test. IL‐1β data were analysed using one‐way anova, followed by SNK, and the t‐test was used for intragroup comparison. The significance level was set at 5%.
Results
In the absence of LPS, only AH Plus and Sealer 26 showed a reduction in cell density, while in the presence of LPS, Sealer 26 had the lowest density compared to the other groups. In terms of mitochondrial activity, at 24 and 48 h, Sealer Plus BC had significantly higher mean values than Sealer 26 and AH Plus (p < .05). Sealer 26 exhibited the lowest levels of oxidative stress and IL‐1β and TNF‐α expression, regardless of the presence of LPS (p < .05).
Conclusions
Although all sealers interfere with the response of macrophages to LPS, contact with epoxy resin‐based sealers can impair cell activity in vitro, while bioceramic sealer seems to favour the inflammatory functions of these cells. |
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AbstractList | AimTo evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS).MethodologyAfter initial setting, the sealers were conditioned with serum‐free culture medium for 24 h (1 ml/cm2). Macrophages from the RAW 264.7 strain were exposed to sealer extracts in a 1:16 ratio in a culture medium with or without LPS. Cell morphology, viability, mitochondrial activity, oxidative stress and gene expression of interleukin 1β (IL‐1β) and tumour necrosis factor‐alpha (TNF‐α) were evaluated. Data on mitochondrial activity, oxidative stress and TNF‐α were analysed using a two‐way analysis of variance (anova) test, followed by the Student–Newman–Keuls post‐test. IL‐1β data were analysed using one‐way anova, followed by SNK, and the t‐test was used for intragroup comparison. The significance level was set at 5%.ResultsIn the absence of LPS, only AH Plus and Sealer 26 showed a reduction in cell density, while in the presence of LPS, Sealer 26 had the lowest density compared to the other groups. In terms of mitochondrial activity, at 24 and 48 h, Sealer Plus BC had significantly higher mean values than Sealer 26 and AH Plus (p < .05). Sealer 26 exhibited the lowest levels of oxidative stress and IL‐1β and TNF‐α expression, regardless of the presence of LPS (p < .05).ConclusionsAlthough all sealers interfere with the response of macrophages to LPS, contact with epoxy resin‐based sealers can impair cell activity in vitro, while bioceramic sealer seems to favour the inflammatory functions of these cells. Abstract Aim To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS). Methodology After initial setting, the sealers were conditioned with serum‐free culture medium for 24 h (1 ml/cm 2 ). Macrophages from the RAW 264.7 strain were exposed to sealer extracts in a 1:16 ratio in a culture medium with or without LPS. Cell morphology, viability, mitochondrial activity, oxidative stress and gene expression of interleukin 1β (IL‐1β) and tumour necrosis factor‐alpha (TNF‐α) were evaluated. Data on mitochondrial activity, oxidative stress and TNF‐α were analysed using a two‐way analysis of variance ( anova ) test, followed by the Student–Newman–Keuls post‐test. IL‐1β data were analysed using one‐way anova , followed by SNK, and the t‐test was used for intragroup comparison. The significance level was set at 5%. Results In the absence of LPS, only AH Plus and Sealer 26 showed a reduction in cell density, while in the presence of LPS, Sealer 26 had the lowest density compared to the other groups. In terms of mitochondrial activity, at 24 and 48 h, Sealer Plus BC had significantly higher mean values than Sealer 26 and AH Plus ( p < .05). Sealer 26 exhibited the lowest levels of oxidative stress and IL‐1β and TNF‐α expression, regardless of the presence of LPS ( p < .05). Conclusions Although all sealers interfere with the response of macrophages to LPS, contact with epoxy resin‐based sealers can impair cell activity in vitro , while bioceramic sealer seems to favour the inflammatory functions of these cells. To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS). After initial setting, the sealers were conditioned with serum-free culture medium for 24 h (1 ml/cm ). Macrophages from the RAW 264.7 strain were exposed to sealer extracts in a 1:16 ratio in a culture medium with or without LPS. Cell morphology, viability, mitochondrial activity, oxidative stress and gene expression of interleukin 1β (IL-1β) and tumour necrosis factor-alpha (TNF-α) were evaluated. Data on mitochondrial activity, oxidative stress and TNF-α were analysed using a two-way analysis of variance (anova) test, followed by the Student-Newman-Keuls post-test. IL-1β data were analysed using one-way anova, followed by SNK, and the t-test was used for intragroup comparison. The significance level was set at 5%. In the absence of LPS, only AH Plus and Sealer 26 showed a reduction in cell density, while in the presence of LPS, Sealer 26 had the lowest density compared to the other groups. In terms of mitochondrial activity, at 24 and 48 h, Sealer Plus BC had significantly higher mean values than Sealer 26 and AH Plus (p < .05). Sealer 26 exhibited the lowest levels of oxidative stress and IL-1β and TNF-α expression, regardless of the presence of LPS (p < .05). Although all sealers interfere with the response of macrophages to LPS, contact with epoxy resin-based sealers can impair cell activity in vitro, while bioceramic sealer seems to favour the inflammatory functions of these cells. Aim To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in macrophage cultures exposed to bacterial lipopolysaccharide (LPS). Methodology After initial setting, the sealers were conditioned with serum‐free culture medium for 24 h (1 ml/cm2). Macrophages from the RAW 264.7 strain were exposed to sealer extracts in a 1:16 ratio in a culture medium with or without LPS. Cell morphology, viability, mitochondrial activity, oxidative stress and gene expression of interleukin 1β (IL‐1β) and tumour necrosis factor‐alpha (TNF‐α) were evaluated. Data on mitochondrial activity, oxidative stress and TNF‐α were analysed using a two‐way analysis of variance (anova) test, followed by the Student–Newman–Keuls post‐test. IL‐1β data were analysed using one‐way anova, followed by SNK, and the t‐test was used for intragroup comparison. The significance level was set at 5%. Results In the absence of LPS, only AH Plus and Sealer 26 showed a reduction in cell density, while in the presence of LPS, Sealer 26 had the lowest density compared to the other groups. In terms of mitochondrial activity, at 24 and 48 h, Sealer Plus BC had significantly higher mean values than Sealer 26 and AH Plus (p < .05). Sealer 26 exhibited the lowest levels of oxidative stress and IL‐1β and TNF‐α expression, regardless of the presence of LPS (p < .05). Conclusions Although all sealers interfere with the response of macrophages to LPS, contact with epoxy resin‐based sealers can impair cell activity in vitro, while bioceramic sealer seems to favour the inflammatory functions of these cells. |
Author | Raucci Neto, Walter Oliveira, Paulo Tambasco Martorano, Antônio Secco Messias, Nadyne Saab Castro Raucci, Larissa Moreira Spinola Bighetti‐Trevisan, Rayana Longo |
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CitedBy_id | crossref_primary_10_1007_s10266_024_00925_1 crossref_primary_10_1007_s00784_024_05593_7 crossref_primary_10_4103_JCDE_JCDE_24_24 crossref_primary_10_1007_s00784_024_05743_x crossref_primary_10_3390_ma16206705 crossref_primary_10_1016_j_surfcoat_2023_129824 |
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To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation... To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation in... Abstract Aim To evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and... AimTo evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation... AIMTo evaluate the effects of AH Plus (Dentsply), Sealer 26 (Dentsply), and Sealer Plus BC (Produtos Médicos e Odontológicos) on cytotoxicity and inflammation... |
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SubjectTerms | biomaterials Cell culture Cell density Cells, Cultured Culture Media Cytology Cytotoxicity Epoxy Resins Gene expression Humans Inflammation Lipopolysaccharides Lipopolysaccharides - pharmacology Macrophages Materials Testing Mitochondria Oxidative stress Root Canal Filling Materials - pharmacology Sealing compounds Silicates - pharmacology Tumor necrosis factor Tumor Necrosis Factor-alpha Tumor necrosis factor-TNF Tumors Variance analysis |
Title | In vitro inflammatory modulation of bioceramic endodontic sealer in macrophages stimulated by bacterial lipopolysaccharide |
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